| 丝素蛋白与丝素蛋白/羟基磷灰石复合材料体外细胞相容性的比较 |
| |
| 引用本文： | 臧志远,王泳,李芳,许云云,古彦铮,李明忠,张学光.丝素蛋白与丝素蛋白/羟基磷灰石复合材料体外细胞相容性的比较[J].苏州大学学报(自然科学版),2010,30(4):705-708,F0003. |
| |
| 作者姓名： | 臧志远 王泳 李芳 许云云 古彦铮 李明忠 张学光 |
| |
| 作者单位： | 臧志远,王泳,李芳,许云云,古彦铮,张学光,ZANG Zhi-yuan,WANG Yong,LI Fang,XU Yun-yun,GU Yan-zheng,ZHANG Xue-guang(苏州大学生物科学技术研究所,江苏苏州,215007);李明忠,LI Ming-zhong(苏州大学材料工程学院,江苏苏州,215021) |
| |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划)资助项目 |
| |
| 摘 要： | 目的探讨丝素蛋白（SF）与丝素蛋白/羟基磷灰石复合材料（SF/HA）的体外细胞相容性,为组织工程提供理想的生物支架材料。方法将小鼠的骨髓间充质干细胞（C3H10T1/2）接种于上述两种生物材料上,运用四唑盐比色试验（MTT法）检测细胞的增殖能力,利用倒置显微镜和扫描电镜观察细胞在材料上的生长形态。结果 C3H10T1/2在SF与SF/HA上都能很好地黏附、生长和增殖。SF、SF/HA和C3H10T1/2都有良好的细胞相容性。结论 3%SF、6%SF、600%SF/HA3组多孔材料的体外细胞相容性最好。
|
| 关 键 词： | 间充质干细胞 丝素蛋白 丝素蛋白/羟基磷灰石复合材料 细胞相容性 |
The Comparative of the Cytocompatibility of Silk Fibroin and Silk Fibroin/Hydroxyapatite Composites |
| |
| ZANG Zhi-yuan,WANG Yong,LI Fang,XU Yun-yun,GU Yan-zheng,LI Ming-zhong,ZHANG Xue-guang.The Comparative of the Cytocompatibility of Silk Fibroin and Silk Fibroin/Hydroxyapatite Composites[J].Suzhou University Journal of Medical Science,2010,30(4):705-708,F0003. |
| |
| Authors: | ZANG Zhi-yuan WANG Yong LI Fang XU Yun-yun GU Yan-zheng LI Ming-zhong ZHANG Xue-guang |
| |
| Affiliation: | 1.Institute of Medical Biotechnology,Soochow University,Jiangsu Suzhou 215007,China;2.School of Materials Engineering,Soochow University,Jiangsu Suzhou 215021,China) |
| |
| Abstract: | Objective To explore the cytocompatibility of the silk fibroin（SF） and SF/hydroxyapatite（HA） with murine mesenchymal stem cell line C3H10T1/2 in vitro.Methods The adherence and growth of the C3H10T1/2 cells on SF and SF /HA was observed by inverted phase contrast microscope and scanning electron microscope.The proliferation of C3H10T1/2 cells on various scaffolds was detected by MTT assay in vitro.Results Both SF and SF /HA could support the adherence,growth and proliferation of C3H10T1/2 cells,which suggested that they have good cytocompatibility and could be used as an ideal biomaterial in tissue engineering.Conlusion 3% SF,6% SF and 600% SF/HA porous materials have the best cytocompatibility in vitro. |
| |
| Keywords: | mesenchymal stem cell silk fbroin silk fibroin/hydroxyapatite cytocompatibility |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
