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混合培养对沼泽红假单孢菌产氢及代谢的影响
引用本文:叶姜瑜,刘婧,廖强.混合培养对沼泽红假单孢菌产氢及代谢的影响[J].环境科学与技术,2009,32(1).
作者姓名:叶姜瑜  刘婧  廖强
作者单位:1. 重庆大学城市建设与环境工程学院
2. 重庆大学动力工程学院,重庆,400044
摘    要:混合培养逐渐引起人们的关注,将沼泽红假单孢菌Rhodopseudomonas palustris和厌氧细菌Enterobacter aerogenes PCM 532的混合培养,并进行生长代谢、产氢及正交实验、Biolog碳源代谢实验。结果表明,混合菌在厌氧光照条件下生长迅速,在好氧黑暗条件下也有一定生长;混合培养的产氢量高于纯培养;正交实验得到,对混合培养产氢影响最大的是底物和混合比例;Biolog微孔板代谢中混合菌可以利用纯菌种培养不能利用的碳源。混合培养表现为协同作用,促进细菌生长和提高产氢能力。

关 键 词:生物制氢  混合培养  Biolog

Hybrid Culture on Impact of Hydrogen Production and Metabolism of Rhodopseudomonas palustris
YE Jiang-yu,LIU Jing,LIAO Qiang.Hybrid Culture on Impact of Hydrogen Production and Metabolism of Rhodopseudomonas palustris[J].Environmental Science and Technology,2009,32(1).
Authors:YE Jiang-yu  LIU Jing  LIAO Qiang
Affiliation:1.School of Urban Construction and Environmental Engineering;Chongqing University;Chongqing 400045;China;2.School of Power Engineering;Chongqing 400044;China
Abstract:Mixed culture gradually aroused people's concern in recent years.Rhodopseudomonas palustris was mixed cultured with Enterobacter aerogenes PCM532 for growth and metabolism,hydrogen production and orthogonal experiment as well as carbon metabolism of Biolog.Results showed that the mixed bacteria grew rapid under the conditions of illumination and anaerobic.Hydrogen production of mixed culture was much higher than pure culture of R.palustris and E.aerogenes.The carbon can be used in mixed bacteria while can n...
Keywords:Biolog
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