不同型别正常人红细胞膜蛋白质组的双向凝胶电泳分析

目的以双向凝胶电泳(2-DE)方法分析不同型别正常人红细胞及细胞膜的蛋白质组图谱。方法提取不同型别的正常人红细胞全细胞蛋白和膜蛋白,以2-DE进行蛋白质的分离,银染显示电泳分离结果,ImageScanner扫描仪扫描并获得图像,以图像分析软件ImageMaster 2D Platinum进行结果分析和比较。结果在不同型别红细胞的全细胞蛋白谱中,有5个蛋白斑点出现表达差异。蛋白硫脲细胞膜处理法与SDS细胞膜处理法获得的红细胞膜蛋白2-DE图谱比较,前者获得的蛋白斑点数增多。结论2-DE有助于分析红细胞膜蛋白质组的表达差异。采用去除膜脂类的样品处理方法,可以提高红细胞2-DE图谱的分辨率。

Analysis of Normal Human Erythrocyte Membrane Proteome of Various Groups by Two-dimensional Gel Electrophoresis

Objective To analyze the proteome map of normal human erythrocyte membrane of various groups by two-dimensional gel electrophoresis (2-DE).Methods Extract the whole cell protein and membrane protein of normal human erythrocytes of various groups for protein separation by 2-DE.Display the result of 2-DE by silver staining and scan the profile by ImageScanner.Analyze the obtained map by ImageMaster 2D Platinum software.Results The differential expression of 5 spots were observed on the whole cell protein map of erythrocytes of various groups.The number of spots on the 2-DE profile of erythrocyte membrane protein obtained by treatment with thiourea was larger than that with SDS.Conclusion 2-DE was helpful to analysis of difference in expression of erythrocyte membrane proteome.The delipidation of samples may increase the resolving power of 2-DE profile of erythrocytes.