二元包装过氧乙酸杀菌效果和储存稳定性试验观察

目的二元包装过氧乙酸是由A液(冰醋酸、硫酸)与B液(过氧化氢)混合后反应生成,观察其对细菌杀灭效果和储存稳定性。方法采用悬液定量杀菌试验和理化分析方法进行了检测。结果以含量100 mg/L的过氧乙酸作用2 min,对大肠杆菌和金黄色葡萄球菌平均杀灭对数值均>5.00;含量200 mg/L的过氧乙酸作用1 min,对白色念珠菌平均杀灭对数值均>5.00;含量2000 mg/L的过氧乙酸作用20 min,对枯草杆菌黑色变种芽孢平均杀灭对数值均>5.00。该二元包装过氧乙酸A、B液分别装入密闭塑料瓶内,经54℃密封储存14 d,与储存前比较,合成后过氧乙酸含量下降率为1.30%。结论本二元包装过氧乙酸消毒剂,杀菌效果好,储存稳定。

EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND STORING STABILITY OF PERACETIC ACID IN BINARY PACKING

Objective To observe the bactericidal efficacy and storing stability of peracetic acid in binary packing,which was produced by mixing solution A(glacial acetic acid,sulfuric acid) and solution B(hydrogen peroxide).Methods Suspension quantitative germicidal test and physicochemical analysis were used for examination.Results The average killing logarithm values of Escherichia coli and Staphylococcus aureus exposed to the solution containing peracetic acid 100 mg/L for 2 min were >5.00,that of Candida albicans exposed to the solution containing peracetic acid 200 mg/L for 1 min was >5.00 and that of spores of Bacillus subtilis var.niger exposed to the solution containing peracetic acid 2000 mg/L for 20 min was >5.00.After the solutions A and B of the peracetic acid in binary packing filled separately in air-tight plastic bottles were stored at 54℃ for 14 days,the peracetic acid content produced decreased by 1.30% as compared with before storing.Conclusion The peracetic acid disinfectant in binary packing has good germicidal efficacy and storing stability.