PRiME HLB固相萃取柱结合气相色谱-串联质谱法快速测定茶叶中200种农药残留

目的建立PRiMEHLB固相萃取柱结合气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)快速测定茶叶中200种农药残留量的分析方法。方法样品用乙腈提取,经PRiME HLB通过式固相萃取柱净化,残留量采用气相色谱-串联质谱法进行检测。结果200种农药在各自质量浓度范围内均呈现良好线性关系,相关系数(r²)均大于等于0.995,方法定量限在0.01~0.05mg/kg之间。在阴性茶叶中加入低、中、高3个添加水平,平均加标回收率及相对标准偏差分别为70.0%~129.2%、1.0%~23.7%。结论该方法操作便捷、快速、准确性强,适合于茶叶中农药多残留高通量的筛查。

Rapid determination of 200 kinds of pesticide residues in tea by PRiME HLB solid phase extraction column combined with gas chromatography-tandem mass spectrometry

Objective To establish a method for the rapid determination of 200 kinds of pesticide residues in tea by PRiME HLB solid phase extraction column combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The sample was extracted with acetonitrile, purified by PRiME HLB solid phase extraction column, and the residues were detected by gas chromatography-tandem mass spectrometry. Results Two hundred kinds of pesticides showed good linear relationships in their respective mass concentration range, the correlation coefficients (r2) were greater than or equal to 0.995, and the limits of quantification of the method were 0.01?0.05 mg/kg. Adding low, medium and high addition levels to negative tea, The average recoveries and relative standard deviations were 70.0%?129.2% and 1.0%?23.7% respectively. Conclusion This method is easy to operate, rapid and has strong accuracy, which is suitable for high-throughput screening of pesticide multi-residues in tea.