| 心复康联合骨髓间充质干细胞移植对濒死心肌的保护作用 |
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| 引用本文： | 李广斌,苏金玲,姜希娟,范英昌.心复康联合骨髓间充质干细胞移植对濒死心肌的保护作用[J].天津中医药,2010,27(2):147-149. |
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| 作者姓名： | 李广斌 苏金玲 姜希娟 范英昌 |
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| 作者单位： | 天津中医药大学基础医学院,天津,300193 |
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| 基金项目： | 天津市卫生局中医、中西医结合科研基金 |
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| 摘 要： | 目的]探讨中药心复康联合骨髓间充质干细胞(BMSCs)移植在大鼠急性心肌梗死后对濒死心肌的保护作用及其机制。方法]经密度梯度离心联合贴壁筛选法培养并纯化BMSCs,采用冠状动脉结扎法建立大鼠急性心肌梗死模型,移植前以5-溴脱氧尿核苷(Brdu)对移植细胞进行标记,术后采用中药心复康灌胃联合干细胞移植治疗。治疗后2周处死动物,免疫组织化学方法检测心肌损伤区Brdu阳性移植细胞数、CD34+新生血管数和细胞增殖核抗原(PCNA)阳性心肌细胞数量。结果]心复康联合干细胞移植治疗组移植细胞存活率增加(与细胞移植组比较P0.05),梗死边缘区新生血管数量及PCNA阳性心肌细胞数量均有所增加(与细胞移植组比较P0.05)。结论]中药心复康联合干细胞移植对大鼠急性心肌梗死后濒死心肌细胞有明显的保护作用,其机制可能与心复康联合干细胞移植能更有效的促进损伤区血管新生有关。
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| 关 键 词： | 心复康 骨髓间充质干细胞 心肌梗死 血管新生 |
| 收稿时间： | 2009/8/20 0:00:00 |
Protective action on impending death cardiac muscles by the therapy of traditional Chinese medicine XinFuKang combined with transplanted bone mesenchymal stem cells |
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| LI Guang-bin,SU Jin-ling,JIANG Xi-juan.Protective action on impending death cardiac muscles by the therapy of traditional Chinese medicine XinFuKang combined with transplanted bone mesenchymal stem cells[J].Tianjin Journal of Traditional Chin Medicine,2010,27(2):147-149. |
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| Authors: | LI Guang-bin SU Jin-ling JIANG Xi-juan |
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| Affiliation: | LI Guang-bin,SU Jin-ling,JIANG Xi-juan (Tianjin University of Traditional Chinese Midicine,Tianjin 300193,China) |
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| Abstract: | Objective]To discuss the protective action on impending death cardiac muscles by the therapy of traditional Chinese medicine XinFuKang(XFK) combined with transplanted Bone mesenchymal stem cells (BMSCs) after rats acute myocardial infarction. Methods] BMSCs were cultured and purified by gradient centrifugation -adhesive method. The rats with acute myocardial infarction were builted by coronary artery ligation. Before transplation,BMSCs were signed by Brdu,and after the OPS. of coronary artery ligation,the... |
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| Keywords: | XinFuKang BMSCs Myocardial infarction Vessel regeneration |
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