骨髓增生异常综合征患者CD34、CD95的表达及临床意义

目的研究骨髓增生异常综合征（MDS）患者骨髓CD34^＋细胞比率及CD34^＋细胞CD95（Fas）表达率并探讨其临床意义。方法应用流式细胞术检测26例MDS患者骨髓单个核细胞（BMMNC）中CD34^＋细胞比率及CD34^＋细胞Fas表达率。结果MDS患者CD34^＋细胞比率RAEB、RAEB—T组较RA组、正常对照组显著增高（P〈0．01）；RAEB、RAEB-T组间比较差异有显著性（P〈0．01）；RA组与正常对照组之间则无显著性差异（P〉0．05）。CD34^＋细胞Fas表达率RA、RAEB、RAEB—T组均较正常对照组显著增高（P〈0．01）；RA与RAEB、RAEB—T组间比较差异有显著性（P〈0．01）；RAEB与RAEB—T组间比较则无显著性差异（P〉0．05）。结论CD34^＋细胞比率进行性升高系提示MDS病情进展的标志；CD34^＋细胞Fas表达率在MDS显著增高但于疾病恶性转化过程中其表达率逐渐降低；细胞增殖与凋亡异常可能系MDS主要发病机制之一。

Expression of CD34 and CD95 in patients with myelodysplastic syndrome and their clinical significance

Objective To study the bone marrow CD34^＋ cell ratio and expression of CD95 （Fas） of CD34^＋ cells in patients with myeledysplastic syndrome （MDS） and to explore their clinical significance. Methods The ratio of CD34 ^＋ cells in bone marrow mononuelear cells（BMMNC） and CD34^＋ cell Fas expression in 26 patients with MDS were detected by using flow cytometry along with labelled CD34 and CD95 monoclonal antibodies. Results The ratio of CD34^＋ cells in patients with MDS-RAEB and RAEB-T were much higher than that in RA and control group （ P 〈 0. 01 ）. There was a significant difference between RAEB and RAEB-T group（ P 〈 0.01 ）, but there was no difference between RA and control group （ P 〉 0.05 ）. The expression of Fas of CD34^＋ cell in RA, RAEB and RAEB-T group significantly increased compared with that in the control group （ P 〈 0.01 ）. The CD34^＋ cell＇ s Fas expression in RAEB and RAEB-T group were significantly higher than that in RA group（ P0.01 ）, while there was no significant dif- ference （ P 〉 0.05） between RAEB and RAEB-T group. Conclusion The increased ratio of CD34^＋ cells in MDS patients may be a indicator of disease progression. The expression of Fas of CD34 ^＋ cells significantly increase in MDS patients and gradually decrease in the process of malignant transformation. Abnormal cell proliferation and apoptosis may be a possible principal pathogenesis of MDS.