坏死性胰腺炎时某些粘附分子表达对中性粒细胞在肺脏聚集的影响

目的探讨急性出血坏死性胰腺炎（ＡＮＰ）时中性粒细胞（ＰＭＮ）聚集于肺脏的机制。方法在ＡＮＰ并发急性肺损伤（ＡＬＩ）模型，采用免疫组化技术等方法，动态观察ＡＮＰ后大鼠肺脏实质细胞细胞间粘附分子１（ＩＣＡＭ１）、ＰＭＮ表面巨噬细胞分化抗原１（ＣＤ１１ｂ／ＣＤ１８）的表达改变和ＰＭＮ肺脏聚集的变化。结果ＡＮＰ的肺脏ＩＣＡＭ１表达逐渐增加，与ＰＭＮ肺脏聚集程度呈显著正相关，而ＰＭＮ表面ＣＤ１１ｂ／ＣＤ１８在ＡＮＰ后１小时即迅速表达至高峰，并持续２４小时。结论ＡＮＰ后，ＰＭＮ在肺脏的大量聚集是建立在ＰＭＮ表面ＣＤ１１ｂ／ＣＤ１８与肺实质细胞ＩＣＡＭ１高表达的基础之上，而ＩＣＡＭ１为这一粘附过程中的可变因素，是影响ＰＭＮ粘附并聚集于肺脏的主要因素。

Effects of cell adhesion molecules expression on neutrophilic infiltration in lung after necrotizing pancreatitis in rats

Objective To explore the mechanism of polymorphonuclear neutrophil (PMN) infiltration in lung after acute hemorrhagic and necrotizing pancreatitis. Methods Adopting a model of rat ANPinduced acute lung injury (ALI), We observed the dynamical changes of ICAM1 expression in lung tissue and CD11b/CD18 expression on PMN and PMN infiltration in lung tissue. Results The ICAM1 expression in lung gradually increased, which significantly correlated with the PMN accumulation after ANP. PMN expressed CD11b/CD18, which quickly occurred and achieved the peak at 1 hour and later maintained high expression within 24 hours. Conclusion PMN infiltration in lung is based on increased expression of ICAM1 in lung tissue and CD11b/CD18 on PMN after ANP. Furthermore, ICAM1 may be a principal factor in the changes of the PMN infiltration in lung tissue and adhesion between PMN and VEC after ANP.