| 三叶因子2基因真核表达载体的构建 |
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| 引用本文： | 张绍荣,杨晓强,邢锐,宋于刚,陈学清.三叶因子2基因真核表达载体的构建[J].第四军医大学学报,2006,27(1):57-58. |
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| 作者姓名： | 张绍荣 杨晓强 邢锐 宋于刚 陈学清 |
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| 作者单位： | 南方医科大学南方医院消化内科,广东,广州,510515 |
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| 基金项目： | 广东省自然基金课题（010578） |
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| 摘 要： | 目的：构建TFF2-pcDNA3．1真核表达载体．方法：应用体外基因拼装方法合成TFF2，然后TA克隆于pGM—T easy载体中．阳性克隆经测序鉴定．再经双酶切消化后，插入真核表达载体pcDNA3．1上，经酶切鉴定与测序证实．结果：合成的TFF2经TA克隆后，筛选得到的阳性克隆，经测序鉴定，与设计的TFF、2基因序列完全一致．经酶切连接并成功插入pcDNA3．1上．结论：成功构建了TFF2基因的真核表达载体，为以后的胃溃疡研究奠定了基础．
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| 关 键 词： | 真核表达载体 TFF2基因 胃溃疡 |
| 文章编号： | 1000-2790(2006)01-0057-02 |
| 收稿时间： | 2005-04-20 |
| 修稿时间： | 2005-05-23 |
Construction of eukaryotic expression vector of human TFF2 gene |
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| ZHANG Shao-Rong,YANG Xiao-Qiang,XING Rui,SONG Yu-Gang,CHEN Xue-Qing.Construction of eukaryotic expression vector of human TFF2 gene[J].Journal of the Fourth Military Medical University,2006,27(1):57-58. |
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| Authors: | ZHANG Shao-Rong YANG Xiao-Qiang XING Rui SONG Yu-Gang CHEN Xue-Qing |
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| Affiliation: | Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China |
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| Abstract: | AIM: To construct a eukaryotic expression vector of human TFF2 gene. METHODS: We assembled a TFF2 gene in pfu mix reaction system and cloned it to pGM-T easy vector. The positive colonies were first confirmed by restriction enzyme digestion and sequencing and then were inserted to eukaryotic expression vector pcDNA3.1 and verified by enzyme digestion and sequencing. RESULTS: After TA colon of synthetic TFF2, the positive colonies were finally verified by sequencing, which were totally in line with the designed coding sequence of TFF2 gene. CONCLUSION: Eukaryotic expression vector of human TFF2 gene has been successfully constructed, which provides a basis for further investigation of gastric ulcer. |
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| Keywords: | eukaryotic expression vector TFF2 gene gastric ulcer |
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