氯通道在不同生长周期的鼻咽癌细胞迁移中的作用

目的：研究不同生长周期的鼻咽癌低分化上皮细胞（CNE-2Z）的迁移能力及氯通道在迁移过程中所起的作用。 方法： 运用血清饥饿法、化学药物双阻断法、有丝分裂药物阻断及摇落法分别将CNE-2Z细胞同步化至细胞周期的G1、S、M期，流式细胞仪检测其同步化效果。结合应用迁移小室和图像分析法，测定迁移率。台盼蓝活细胞染色法测定药物的细胞毒性。 结果： 不同生长周期的细胞迁移能力不同，G1期细胞迁移能力最强，随后是M期， S期迁移能力最弱。氯通道阻断剂（ATP、NPPB、tamoxifen）抑制CNE-2Z细胞迁移，但不同的氯通道阻断剂对不同生长周期CNE-2Z细胞迁移的阻滞效应不相同。 结论： CNE-2Z细胞的迁移能力与其所在的生长周期密切相关，氯通道在各期CNE-2Z细胞的迁移过程中起着重要作用。

Roles of chloride channels in the migration of nasopharyngeal carcinoma cells at different stages of the cell cycle

AIM: To clarify the migration capability of nasopharyngeal carcinoma cells (CNE-2Z) at different stages of the cell cycle and the roles of chloride channels in cell migration. METHODS: Synchronous cells were obtained by the serum deprivation,double chemical-block, mitotic arrest and shake-off techniques. Cell cycle distribution of CNE-2Z cells was analyzed by the flow cytometry. Migration rate was assayed by transwell chambers and by image analysis. The cytotoxicity of chemicals on cells was tested by MTT assay. RESULTS: CNE-2Z cells at different stages of the cell cycle exhibited different migratory ability. The migration rate of the three stages was G_1>M> S. The migration of CNE-2Z cells was inhibited by chloride channel blockers (ATP, NPPB and tamoxifen), but the inhibitory effect of the blockers varied with cells at different stages. CONCLUSIONS: The migratory ability is associated with the cell cycle in CNE-2Z cells. Chloride channels play an important role in cell migration of CNE-2Z cells.