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阿魏菇多糖的提取、纯化及其组成研究
引用本文:马纪,李轶杰,王龙,胡瑞,张富春.阿魏菇多糖的提取、纯化及其组成研究[J].新疆大学学报(理工版),2002,19(2):219-221.
作者姓名:马纪  李轶杰  王龙  胡瑞  张富春
作者单位:[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]新疆产品质量技术监督检验所,新疆乌鲁木齐830000
摘    要:采用热水浸提、Sevag法去蛋白、乙醇沉淀,冷冻干燥,从阿魏菇(pleurotus ferulae Lenzi)子实体及菌丝球中提取阿魏菇粗多糖(PFP), 苯酚-硫酸法测定多糖含量为PFP147.2%,PFP为41.4%,经过Sephadex200层析柱分离和薄层层析分析,可知阿魏菇多糖是由三种单糖组成的均一杂多糖。

关 键 词:阿魏菇  多糖  提取  纯化  组成分析  含量测定  中药  苯酚-硫酸法
文章编号:1000-2839(2002)02-0219-03
修稿时间:2001年7月19日

Studies on the Isolation, Purification And Composition of Polysaccharide from Pleurotus ferulae
MA Ji ,LI Yi jie ,WANG Long ,HU Rui ,ZHANG Fu chun.Studies on the Isolation, Purification And Composition of Polysaccharide from Pleurotus ferulae[J].Journal of Xinjiang University(Science & Engineering),2002,19(2):219-221.
Authors:MA Ji  LI Yi jie  WANG Long  HU Rui  ZHANG Fu chun
Affiliation:MA Ji 1,LI Yi jie 1,WANG Long 2,HU Rui 1,ZHANG Fu chun 1
Abstract:The polysaccharide of Ferule Mushroom from its fruiting body (PFPf) and mycelium (PFPm) by boiling in water and precipitation in ethyl alcohol were obtained. The contents of PFPf1 was 47.2% and PFPm1 was 41.4% in phenol vitriol method. It was found out that PFPf, composed of three different kinds of sugar, was a single component polysaccharide by using the method of column chromatography and thin layer chromatography.
Keywords:Ferule Mushroom  polysaccharide isolation  purification  composition
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