基于高分辨质谱数据库的黄连炮制前后生物碱变化规律

目的 构建黄连Coptis chinensis生物碱类成分的高分辨质谱数据库(HR-MS-Database),研究不同炮制方法炮制前后黄连生物碱类化学成分的变化。方法 采用超高效液相色谱-四极杆-飞行时间质谱联用(UPLC/Q-TOF-MS)技术手段,借助SCIEX公司LibraryView数据库平台,建立黄连生物碱类成分高分辨质谱数据库,并对不同黄连炮制品中炮制前后的生物碱类成分含量进行测定和差异分析。结果 建立黄连生物碱类成分高分辨质谱数据库,包含黄连生物碱类成分31种;对姜黄连、酒黄连、萸黄连及黄连生品4种样品测定结果显示,黄连炮制前后生物碱成分差异明显。黄连经姜炙后,药根碱、表小檗碱、非洲防己碱含量略有上升,黄连碱、小檗碱含量略有下降,巴马汀含量未见改变;萸黄连中药根碱、表小檗碱、小檗碱含量下降,黄连碱、巴马汀及非洲防己碱含量未见变化;而酒黄连中所测定的6种生物碱含量均显著提高(P<0.05)。结论 建立了一种黄连炮制前后生物碱类成分变化规律的快速识别方法,并定量了在黄连炮制前后含量变化显著的6种生物碱,为黄连炮制品的药效物质基础与质量控制标准研究提供参考。

Changing regularity of alkaloids from Coptis chinensis before and after processing based on HR-MS-Database

Objective To establish high-resolution mass spectrometry database (HR-MS-Database) of alkaloid constituents fromCoptis chinensis,and explore alkaloids variation ofC.chinensis treated by different processing methods.Methods Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to create HR-MS-Database of alkaloid constituents fromC.Chinensis by means of LibraryView database platform of SCIEX Corporation.And content determination and difference analysis of the alkaloids fromC.chinensis before and after processing were conducted.Results The HR-MS-Database of alkaloid constituents fromC.chinensis containing 31 species of alkaloids was built.The determination results showed that the composition of alkaloids in four types ofCoptidis Rhizoma(Huanglian) pieces (ginger juice stir-fried pieces,wine stir-fried pieces,evodiae juice stir-fried pieces,crude pieces) was obviously different.The contents of jatrorrhizine,epiberberine and columbamine in Coptidis Rhizoma pieces slightly increased by ginger juice stir-fried processing,but the contents of coptisine and berberine were slightly decreased,and the content of palmatine remained unchanged;contents of jatrorrhizine,epiberberine and berberine in evodiae juice stir-fried pieces were decreased,yet contents of coptisine,palmatine,columbamine still stayed stable.Moreover,the contents of six alkaloids (jatrorrhizine,epiberberine,berberine,coptisine,palmatine and columbamine) in wine stir-fried pieces obviously improved (P<0.05).Conclusion The study will provide an effective method for fast identification of alkaloid variation.It was found that the contents of six species of alkaloids fromC.chinensis changed significantly before and after processing,which provided a reference for their pharmacodynamic material basis and quality control standard of processedRhizoma Coptis.