中药复方筋脉通对链脲佐菌素诱导糖尿病大鼠肝脏损伤和脂肪变性的保护作用

目的初步探讨中药复方筋脉通对链脲佐菌素(streptozotocin,STZ)诱导的1型糖尿病(type 1 diabetes mellitus,T1DM)大鼠肝脏损伤和脂肪变性的保护作用。方法将24只SD大鼠随机分为糖尿病组、筋脉通组和正常对照组,每组各8只。采用单次腹腔注射STZ的方法建立T1DM大鼠模型,成模后灌胃12周,筋脉通组灌胃中药复方筋脉通,糖尿病组和正常对照组灌胃等量的生理盐水,并监测血糖、体重变化。12周后测定血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)和游离脂肪酸(free fatty acid,FFA)表达,进行HE染色观察病理学改变,计算非酒精性脂肪性肝病活动评分,Masson染色观察肝纤维化情况。结果与正常对照组比较,糖尿病组大鼠血清ALT、AST、TG、FFA水平明显升高,肝/体重的比值增加,肝组织可见小泡性脂肪变性,伴炎症浸润、部分坏死,非酒精性脂肪性肝病活动评分显著增加,未见明显气球样变和纤维化。筋脉通可显著改善血清肝功能异常和脂代谢紊乱,减轻肝细胞脂肪变性及炎症,降低NAS评分。结论筋脉通对于STZ诱导糖尿病大鼠肝脏损伤和脂肪变性具有保护作用。

Protective effects of Jinmaitong on liver injury and hepatic steatosis in T1DM rats induced by streptozotocin

Objective To investigate the protective effect of Jinmaitong on liver injury and hepatic steatosis in type 1 diabetes mellitus(T1DM)rats induced by streptozotocin(STZ).Methods 24 sprague dawley(SD)rats were randomly divided into diabetic group,Jinmaitong group and normal group,8 in each group.The T1DM rat model was established by single intraperitoneal injection of STZ.After mold formation,12 weeks of gavage,the Jinmaitong group was gavaged by Chinese medicine compound Jinmaitong,the diabetic group and the normal group were given the same amount of normal saline,and blood sugar and changes of body weight of rats were detected.The alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triglyceride(TG)and free fatty acid(FFA)in serum were measured after 12 weeks.The pathological changes were observed by HE staining.Nonalcoholic fatty liver disease activity score(NAS)was calculated and liver fibrosis was observed by Masson staining.Results Compared with the normal group,the levels of ALT,AST,TG,FFA increased significantly in the diabetics group,and the liver/body weight ratio were higher significantly,vesicular steatosis was observed in the liver tissue,accompanied by inflammatory infiltration and partial necrosis,the NAS score was significantly increased,and no obvious ballooning and fibrosis were observed.Jinmaitong can significantly improve serum liver dysfunction and lipid metabolism,reducing hepatic steatosis and inflammation,decreasing NAS score.Conclusion Jinmaitong has the protective effect on liver injury and hepatic steatosis in T1DM rats induced by STZ.