激光扫描共聚焦光谱系统设计

为提高激光扫描共聚焦显微镜光谱模块的分光性能,设计了双Amici棱镜光谱结构.根据棱镜色散特性,计算得到Amici棱镜和双Amici棱镜的线性与非线性数学模型,给出直视型棱镜结构参数.比较相同色散条件下Amici和双Amici棱镜结构,得到双Amici棱镜组能提供更好的色散线性度.在光谱波段400～700 nm,中心波...

Design of laser scanning confocal spectroscopy system

A spectral structure for the double Amici prism was designed to improve the spectroscopic performance of a laser scanning confocal microscope spectroscopy module.Linear and nonlinear mathematical models were formulated for the Amici and double Amici prisms,and the structure parameters of directviewing prism were chosen according to the prism dispersion characteristics.The structures of the Amici and double Amici prisms were compared for the same dispersion conditions,and the double Amici prism group was found to provide better dispersion linearity.A common optical axis direct vision spectroscopic system was developed using a double Amici prism for a 400-700 nm spectral range and 5 nm spectral resolution,with central wavelength deviation angle of 0°and maximum dispersion angle of 4°.The experimental results show that the dispersion linearity of this structure is significantly better than that of a single prism.Spectral resolution issues caused by the nonlinear spectroscopic characteristics of prisms are greatly reduced.