维生素E对癫痫大鼠认知功能障碍的治疗作用及其可能机制

目的：探讨维生素E（VitE）对癫痫大鼠认知功能障碍的治疗作用及其可能机制。方法：将30只成年雄性SD大鼠随机分为健康对照组、单纯致痫组（SE组）、VitE按体重100mg／（kg·d）]干预组（VitE组）。采用Morris水迷宫实验方法检测致癫后大鼠学习记忆功能变化，同时检测脑组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—PX）、谷胱甘肽（GSH）、丙二醛（MDA）的水平。结果：（1）SE组大鼠寻找平台的潜伏期明显长于对照组，具有统计学意义（P〈0．05），VitE组寻找平台的潜伏期相对于SE组显著缩短（P〈0．05）。撤离平台后，SE组大鼠在平台所在象限的停留时间明显短于对照组（P〈0．05），VitE治疗后大鼠在平台所在象限的停留时间较SE组显著延长（P〈0．05）。（2）SE组SOD、GSH—PX、GSH显著下降，MDA明显增高，VitE干预组SOD、GSH．PX-GSH显著增高，而MDA明显下降，具有统计学意义（P〈0．05）。结论：VitE可改善癫痫持续状态后大鼠认知功能，其可能机制是通过减轻海马区的氧化应激反应减轻海马区的损伤，从而实现改善认知功能。

Vitamin E for Cognitive Dysfunction of Rats with Epilepsy

Objective： The purpose of the study was to investigate the effects of vitamin E （VitE） on cognitive deficits induced by status epilepticus （SE） and the possible mechanism in terms of oxidative stress in hippocampus. Methods： A total of 30 male Sprague-Dawley rats （200± 20 g） were randomly assigned to one of the following groups： （n =10 in each group）： Control group,SE group and Vit E group. Severe cognitive deficits in lithium-pilocarpine seizure model was tested by the water maze task. Glutathione （GSH） and Maleic Dialdehyde（MDA） content of the homogenate of Hippocampus were measured; The activity of Superoxide Dismutase （SOD） and glutathione peroxidase （GSH-PX）in the homogenate of Hippocampus were detected. Results： Vitamin E could attenuate SE-induced cognitive deficits. Biochemical experiments revealed that SE can significantly increase content of MDA, decrease content of GSH and decreased the activities of SOD and GSH-PX in the homogenate of Hippocampus, which can be reversed by VitE. Conclusions： Vit E exhibits therapeutic potential for SE-induced cognitive deficits, which is most likely related, at least in part, to its anti-oxidative stress actions in hippocampus.