Separation and properties of an H2B histone variant from the sperm chromatin of the sea urchin Sphaerechinus granularis |
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| Authors: | V Giancotti F Quadrifoglio M Lancieri G Geraci |
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| Affiliation: | 1. Istituto di Chimica, Università degli Studi di Trieste, Italy;2. Istituto di Biologia Generale e Genetica Università degli Studi di Napoli, Napoli, Italy;3. Laboratorio di Embriologia Molecolare, CNR, Arco Felice, Napoli, Italy |
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| Abstract: | The purification and the physico-chemical characterization of one of the two H2B histone variants from the sperm of the sea urchin Sphaerechinus granularis are reported. The molecule shows, in addition to a distinctive molecular weight value, an amino acid composition different both from that of calf thymus H2B histone and from those of H2B histones from chromatin of sperm and embryos of other sea urchins. Circular dichroism and fluorescence data are discussed in comparison to those of calf thymus H2B. |
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