| 日本血吸虫成虫67kD蛋白的分离与鉴定 |
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| 引用本文： | 袁仕善,曾宪芳,黄跃龙,王敏,周东明,易新元.日本血吸虫成虫67kD蛋白的分离与鉴定[J].中南大学学报(医学版),2002,27(2):102-104. |
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| 作者姓名： | 袁仕善 曾宪芳 黄跃龙 王敏 周东明 易新元 |
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| 作者单位： | 中南大学湘雅医学院血吸虫病研究室,长沙,410078 |
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| 基金项目： | 湖南省科技厅资助项目(0 0jzy2 115 ) |
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| 摘 要： | 目的 :分离日本血吸虫感染及免疫血清识别的成虫抗原 (AWA)中的特异蛋白带 ,为血吸虫病免疫诊断提供新的抗原分子。方法 :免疫印迹法分析AWA的特异蛋白带 ,电泳层析法分离靶抗原。结果 :获得了感染血清和免疫血清识别的 6 7kD蛋白。结论 :电泳层析法是一种分离血吸虫抗原的有效方法
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| 关 键 词： | 日本血吸虫 成虫抗原 免疫印迹法 电泳 67kD蛋白 |
| 文章编号： | 1000-5625(2002)02-0102-03 |
| 修稿时间： | 2001年7月13日 |
Fractionation and identification of Schistosoma japonicum adult worm 67kD protein |
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| YUAN Shi shan,ZENG Xian fang,HUANG Yue long,et al..Fractionation and identification of Schistosoma japonicum adult worm 67kD protein[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2002,27(2):102-104. |
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| Authors: | YUAN Shi shan ZENG Xian fang HUANG Yue long |
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| Affiliation: | (Laboratory of Schistosomiasis, Xiangya School of Medicine,Central South University,Changsha,410078, China) |
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| Abstract: | Objective To isolate the specific protein bands which react to sera from rabbits infected with Schistosoma japonicum(Sj)and those immunized with Sj adult worm antigens (AWA) for immunodiagnosis of schistosomiasis. Methods AWA was analysed using SDS PAGE and immunoblotting, and 67kD protein was fractionated by electrophoretic chromatography. Results 67kD protein which reacted to rabbit sera infected with Sj and immunized with AWA was obtained after the fractionation of AWA through electrophoretic chromatography. Conclusions Electrophoretic chromatography is a useful tool for the fractionation of schistosome antigens. |
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| Keywords: | Schistosoma japonicum adult worm antigens * immunoblotting electrophoresis 67kD protein * |
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