HPLC—ELSD法测定肝速康胶囊中齐墩果酸的含量

目的：建立HPLC—ELSD法测定肝速康胶囊中齐墩果酸的含量。方法：采用Phenomenex C18色谱柱，以甲醇-0．1％三氟醋酸（TFA）溶液（90：10）为流动相，柱温为室温，流速为1．0mL·min^-1，以蒸发光散射检测器检测，漂移管温度88℃，空气作为载气，流速为1．6L·min^-1。结果：齐墩果酸在2．007～12．04μg与相应峰面积呈良好线性关系（r=0．9992，n=5），平均回收率为100．12％，RSD为0．62％（n=5）。结论：方法准确，专属性强，该法可用于该制剂的质量控制和评价。

Determination of Oleanolic Acid in Gansukang Capsules by HPLC-ELSD

Objective： An HPLC-ELSD was developed and validated to the determination of oleanolic acid in Gansukang Capsules. Methods： the determination was performed on Phenomenex C18 column with the mobile phase consisted of methanol - 0. 1% trifluoroacetie acid （TFA） solution （90：10） at ambient temperature. ELSD was used as detector , the drift tube temperature was 88℃ and eluant gas was air with the flow rate was 1.6L·min^-1. Results： The method showed a good linear relationshi Pwithin the range of oleanolic acid was 2. 007 - 12. 04μg （r = 0. 999 2, n = 5 ） . The mean recovery was 100. 12% with RSD = 0. 62% （n = 5 ） . Conclusion： The method is simple and sensitive and can be used for the quality control of Gansukang Capsules, RSD = 1.31%.