| 正交试验法优选苦丁茶老叶中熊果酸的提取工艺 |
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| 引用本文： | 蒙大平,路玫,黄远霞,黄卓坚.正交试验法优选苦丁茶老叶中熊果酸的提取工艺[J].中国药房,2007,18(6):422-424. |
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| 作者姓名： | 蒙大平 路玫 黄远霞 黄卓坚 |
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| 作者单位： | 1. 广西壮族自治区人民医院,南宁市,530021
2. 桂林医学院,桂林市,541004 |
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| 基金项目： | 广西科学研究与技术开发计划项目 |
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| 摘 要： | 目的:优选苦丁茶老叶中熊果酸的提取工艺条件。方法:以熊果酸含量为考察指标,采用L9(34)正交试验法考查不同因素和水平对熊果酸提取工艺的影响。结果:影响工艺的因素次序为提取次数>液固比>乙醇浓度>提取时间;最佳提取工艺为在85℃水浴温度下,液固比为16∶1、乙醇浓度为80%、水浴提取2次、每次2.5h。在此条件下,熊果酸的含量可达45.65μg.g-1。结论:该提取工艺合理、可行。
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| 关 键 词： | 正交试验法 苦丁茶 熊果酸 提取工艺 |
| 文章编号： | 1001-0408（2007）06-0422-03 |
| 收稿时间： | 2006-05-24 |
| 修稿时间： | 2006-09-15 |
Optimizing Extraction Technology of Ursolic Acid in Ilex Kudingcha. C.J Tseng by Orthogonal Experiment |
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| MENG Daping,LU Mei,HUANG Yuanxia,HUANG Zhuojian.Optimizing Extraction Technology of Ursolic Acid in Ilex Kudingcha. C.J Tseng by Orthogonal Experiment[J].China Pharmacy,2007,18(6):422-424. |
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| Authors: | MENG Daping LU Mei HUANG Yuanxia HUANG Zhuojian |
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| Abstract: | OBJECTIVE: To optimize the extraction technology of ursolic acid from the old leaves of Ilex Kudingcha. C. J Tseng. METHODS: With the content of Ursolic acid used as index, orthogonal experiment was carried out to study the influence of different factors and levels on the ursolic acid extraction. RESULTS: The sequence of primary factors influencing extraction technique was as follows: extraction times > the ratio of liquid to solid > concentration of ethanol > extraction time. The optimum extraction technology was as follows: water-bath temperature at 85℃ , ratio of liquid to solid at 16∶ 1, concentration of Ethanol at 80% , extraction for 2 times and 2. 5 hours each time. In this condition, the extraction rate of ursolic acid was 45. 65μ g· g-1. CONCLUSION: The extraction technology is appropriate and feasible. |
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| Keywords: | Orthogonal experiment Ilex Kudingcha C J Tseng Ursolic acid Extraction technology |
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