阳桃胚乳愈伤组织诱导和不定芽发生的研究

首次成功建立阳桃胚乳组织培养并获得胚乳再生植株。胚乳愈伤组织诱导以培养基MS 2,4-D2．0mgL^-1 BA0．2mgL^-1的效果最好，诱导频率可达94．7％，愈伤组织乳白色，结构致密，生长旺盛；将其接种在培养基MS ZT3．0mgL^-1 NAA0．2mgL^-1上，愈伤组织由乳白色致密型转变为淡绿色致密型，进而形成绿色芽点，分化出不定芽，分化频率可达73．3％；胚乳植株在培养基MS ZT2．0-2．5mgL^-1 NAA0．05mgL^-1上进行壮苗和营养繁殖。

Callus Induction and Plant Regeneration from Endosperm of Averrhoa carambola

Explants from endosperm of Averrhoa carambola were used to establish culture in vitro and regeneration system. The experiments showed that endosperm inoculated on MS medium supplemented with 2.0 mg L-1 2,4-D and 0.2 mg L-1 BA, gave highest callus induction rate (94.7%). The callus was milk white and compact. When the endosperm derived callus was cultured on MS medium containing 3.0 mg L-1 ZT and0.2 mg L-1 NAA, the callus became greenish and compact, the shooting frequency being 73.3%. Optimal medium for multiplication of plantlets was MS supplemented with 2.0-2.5 mg L-1 ZT and 0.05 mg L-1 NAA.