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| 非接触式扫描离子电导显微镜技术在探测活体细胞表面微结构中的应用 | |
| 引用本文： | 杨茜,刘晓,马丽颖,李长峰,鲍亚盟,高瑞玲,张西正,张彦军.非接触式扫描离子电导显微镜技术在探测活体细胞表面微结构中的应用[J].电子显微学报,2009,28(5):489-494. |
| 作者姓名： | 杨茜 刘晓 马丽颖 李长峰 鲍亚盟 高瑞玲 张西正 张彦军 |
| 作者单位： | 1. 国家纳米技术与工程研究院,天津,300457 2. 军事医学科学院卫生装备研究所,天津,300161;天津国家生物防护装备工程技术研究中心,天津,300161 3. 国家纳米技术与工程研究院,天津,300457;军事医学科学院卫生装备研究所,天津,300161;天津国家生物防护装备工程技术研究中心,天津,300161 |
| 基金项目： | 国家自然科学基金重点项目，天津市应用基础及前沿技术研究计划 |
| 摘 要： | 扫描离子电导显微镜技术是在纳米尺度进行非导电的生物样品成像的一种新型扫描探针显微镜技术。通过成功制备扫描离子电导显微镜扫描探测用纳米尺度玻璃微探针,对其进行了功能性评估;而后通过绘制探针-样品接近曲线,阐述了扫描离子电导显微镜技术实现非接触高分辨率探测的工作原理;最后采用该显微镜技术对导电标准样品及活体肾上皮A6细胞进行了表面形貌扫描成像,并与A6细胞表面形貌的扫描电镜图像进行了对照。结果表明,扫描离子电导显微镜技术不仅可实现导电样品的扫描成像,而且适宜于在生理条件下、非接触式地研究活体细胞表面的三维形貌,从而为人们深入研究细胞表面微观结构与生理功能提供了全新的技术手段。 |
| 关 键 词： | 扫描离子电导显微镜技术 纳米生物学 肾上皮细胞 |
Non-contact scanning ion conductance microscopy for high-resolution morphological imaging of live cells |
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| YANG Xi,LIU Xiao,MA Li-ying,LI Chang-feng,BAO Ya-meng,GAO Rui-ling,ZHANG Xi-zheng,ZHANG Yan-jun.Non-contact scanning ion conductance microscopy for high-resolution morphological imaging of live cells[J].Journal of Chinese Electron Microscopy Society,2009,28(5):489-494. | |
| Authors: | YANG Xi LIU Xiao MA Li-ying LI Chang-feng BAO Ya-meng GAO Rui-ling ZHANG Xi-zheng ZHANG Yan-jun |
| Affiliation: | YANG Xi, LIU Xiao, MA Li-ying, LI Chang-feng, BAO Ya-meng, GAO Rui-ling, ZHANG Xi-zheng, ZHANG Yan-jun ( 1. China National Academy of Nanotechnology and Engineering, Tianjin 300457 ; 2. Institute of Medical Equipment, Military Academy of Medical Science, Tianjin 300161 ; 3. National Bio-Protection Engineering Center, Tianjin 300161 ; China) |
| Abstract: | As a new kind of scanning probe microscopy,scanning ion conductance microscopy (SICM) is designed for imaging non-conducting biological sample at the nanometer scale. Firstly, nanometer scale glass micropipettes were pulled successfully, and were functionally evaluated. Secondly, a micropipette-sample approaching curve was obtained for describing the working principle of SICM. Finally, SICM was used to scan the morphology of a conductive standard sample and live renal epithelial A6 cells. The topography of A6 cells monolayer was verified with Scanning Electron Microscopy (SEM) observation. As a result, non-contact SICM can be used not only for scanning conductive samples, but also for acquiring 30 morphological image of non-conducting living cells. It provides a powerful tool for in-depth studying the relationship between the cell membrane microstructures and their physiological functions. |
| Keywords: | SICM nanobiology renal epithelial cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
