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Effect of green and black teas (Camellia sinensis L.) on the characteristic microflora of yogurt during fermentation and refrigerated storage
Authors:Imène Jaziri  Mourad Ben Slama  Houcine Mhadhbi  Maria C Urdaci  Moktar Hamdi
Affiliation:1. Laboratoire d’Ecologie et de Technologie Microbienne, Institut National de Sciences Appliquées et de Technologie (INSAT), PB. 676, 1080 Tunis, Tunisia;2. Laboratoire de Microbiologie et Biochimie Appliquée, ENITA de Bordeaux, 1, cours du Général de Gaulle, BP 201, 33175 Gradignan, France;3. Unité de Recherche Chimie Physiques des Milieux et Substances Naturels, 05.INRAP.01, Institut National de Recherche et d’Analyse Physico-Chimique (INRAP), Pôle Technologique de Sidi Thabet, 2020 Sidi Thabet, Tunisia;4. Centre National des Sciences et Technologies Nucléaires (CNSTN), Pôle Technologique de Sidi Thabet, 2020 Sidi Thabet, Tunisia
Abstract:The effect of tea on the fermentation and survival of yogurt microorganisms was studied. Green and black teas were added to milk at the beginning of fermentation. Acidity of yogurt products and survival of their microflora were studied during 42 days at 4 °C. Results showed that the presence of tea did not significantly (P < 0.05) influence the yogurt characteristic microorganisms. HPLC studies demonstrated that yogurt bacteria did not affect tea catechins when they were incubated together for 48 h. Indeed, all five products reached about 109 CFU/ml after 6 h of fermentation. Viability during 6 weeks storage at 4 °C varied very little (8.35 < log CFU/ml < 8.65). Similarly, green and black teas had no effect on lactic acid levels of the final products (after 6 weeks of storage, acidity remained above 80 °D). According to these findings, addition of teas or tea catechins to yogurt can be recommended to take advantage of their beneficial properties on human health attributed to their antioxidant and antimicrobial activities.
Keywords:Green tea  Black tea  Yogurt bacteria  Catechins  Functional food
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