| HPLC法测定多烯紫杉醇脂质体药物包封率 |
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| 引用本文： | 吴卫,程怡,吴琼.HPLC法测定多烯紫杉醇脂质体药物包封率[J].辽宁中医杂志,2012(10):2026-2027. |
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| 作者姓名： | 吴卫 程怡 吴琼 |
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| 作者单位： | 广州中医药大学中药学院;桂林医学院 |
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| 基金项目： | 国家自然科学基金资助项目(30772790) |
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| 摘 要： | 目的:建立多烯紫杉醇脂质体药物包封率测定方法。方法:采用HPLC法测定脂质体中多烯紫杉醇的含量及包封率,色谱柱:Diamonsil C18(250mm×4.6 mm,5μm);流动相:乙腈-水(55∶45);柱温:30℃;流速:1.0mL.min-1;紫外检测波长:230nm。结果:多烯紫杉醇在20.4～204μg·mL-1范围内线性关系良好(r=0.9997),平均回收率为97.59%,RSD为1.53%。结论:该方法简单、快速、准确,可用于多烯紫杉醇脂质体包封率的测定。
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| 关 键 词： | 多烯紫杉醇 脂质体 包封率 |
Analysis of Entrapment Efficiency of Docetaxel in Docetaxel Liposomes by HPLC |
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| WU Wei,CHENG Yi,WU Qiong.Analysis of Entrapment Efficiency of Docetaxel in Docetaxel Liposomes by HPLC[J].Liaoning Journal of Traditional Chinese Medicine,2012(10):2026-2027. |
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| Authors: | WU Wei CHENG Yi WU Qiong |
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| Affiliation: | 1(1.School of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong,China; 2.Guilin Medical University,Guilin 541004,Guangxi,China) |
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| Abstract: | Objective:To establish a determination method for entrapment efficiency of docetaxel in docetaxel liposomes.Methods:The content and entrapment efficiency was determined by HPLC.Chromatographic column:Diamonsil C18(250mm×4.6mm,5μm);Mobile phase:acetonitrile-water(55∶ 45);Column temperature:30℃;Velocity:1.0mL·min-1;Detected wavelength:230 nm.Results:The linear range for docetaxel was 20.4~204μg·mL-1(r=0.9997).The average recovery was 97.59% and RSD was 1.53%.Conclusion:This determination method is simple,raipd and accurate,can be used for the analysis of entrapment efficiency of docetaxel in docetaxel liposomes. |
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| Keywords: | docetaxel liposomes entrapment efficiency |
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