| 4种检测超广谱β-内酰胺酶方法的比较 |
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| 引用本文： | 杜军,李敏,崔景荣,朱永红,王玉炯.4种检测超广谱β-内酰胺酶方法的比较[J].宁夏大学学报(自然科学版),2009,30(3):268-270. |
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| 作者姓名： | 杜军 李敏 崔景荣 朱永红 王玉炯 |
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| 作者单位： | 1. 宁夏大学生命科学学院,西部特色生物资源保护与利用教育部重点实验室,宁夏银川,750021
2. 北京大学医学部,北京,100083 |
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| 基金项目： | 国家重点基础研究发展计划"973"项目，高等学校科技创新工程重大项目培育基金资助项目，北京大学天然药物及仿生药物国家重点实验室开放课题资助项目，宁夏大学自然科学研究基金资助项目 |
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| 摘 要： | 以头孢噻肟、头孢他啶、头孢曲松和氨曲南为指示底物,用双纸片协同实验、纸片表型确证实验、三维实验和E-test法4种方法检测45株产超广谱β-内酰胺酶（ESBLs）阳性和59株产ESBLs阴性的大肠埃希菌和肺炎克雷伯菌.结果发现,4种方法的敏感性依次为95.56%,97.78%,93.33%,97.78%,特异性依次为96.61%,100.00%,98.31%,100.00%;头孢噻肟为宁夏地区用于检测ESBLs的最适指示底物;纸片表型确证实验和E-test法是4种检测方法中用于检测产ESBLs较可靠的方法,用三维实验检测产ESBLs菌株适合标本量较少的小型医院,双纸片协同实验和纸片表型确证实验在所有医院都适用.
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| 关 键 词： | 超广谱β-内酰胺酶 大肠埃希菌 肺炎克雷伯菌 |
Comparison of Four Different Methods for Detection of Extended-spectrum Beta-lactamases |
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| Du Jun,Li Min,Cui Jingrong,Zhu Yonghong,Wang Yujiong.Comparison of Four Different Methods for Detection of Extended-spectrum Beta-lactamases[J].Journal of Ningxia University(Natural Science Edition),2009,30(3):268-270. |
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| Authors: | Du Jun Li Min Cui Jingrong Zhu Yonghong Wang Yujiong |
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| Affiliation: | Du Jun , Li Min , Cui Jingrong , Zhu Yonghong , Wang Yujiong (1. School of Life Science, Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China; 2. Health Science Center, Peking University, Beijing 100083, China) |
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| Abstract: | To Detect 45 strains of producing extended-spectrum beta-lactamase （ESBLs） and 59 strains nonproducing ESBLs in Escherichia coli and Klebsiella pneumoniae with double-disc synergy test, disc diffusion confirmatory test, three-dimensional test and E test, ceftazidime,cefotaxime,ceftriaxone and aztreonem were used as indicators. The sensitivity was 95.56% ,97.78% ,93.33% and 97.78% ,the specificity was 96.61% ,100.00%, 98.31%and 100. 00%, respectively. Cefotaxime was considered to be the better indicator. The disc diffusion confirmatory test and E-test were more reliable methods. The double-disc synergy test and disc diffusion confirmatory test were more convenient and suitable for the routine screening in hospital. |
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| Keywords: | extended-spectrum beta-lactamase（ESBLs） Escherichia coli Klebsiella pneumonia |
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