脂联素受体在正常SD大鼠乳鼠心肌细胞的分布和表达

目的通过体外培养SD大鼠乳鼠心肌细胞,研究脂联素受体1(AdipoR1)、脂联素受体2(AdipoR2)及T-钙黏蛋白(T-cadherin)三种脂联素受体在大鼠乳鼠心肌细胞上的分布和表达。方法采用酶消化法原代培养乳鼠心肌细胞,通过α-肌动蛋白免疫荧光法对原代培养的心肌细胞进行鉴定,并在倒置相差显微镜下观察细胞生长状态。选用原代培养96h的单层心肌细胞进行实验,使用RT-PCR和细胞免疫组织化学方法检测AdipoR1、AdipoR2、T-cadherin的mRNA和蛋白在心肌细胞中的表达情况。结果在SD大鼠乳鼠心肌细胞上均检测到了AdipoR1、AdipoR2、T-cadherin的mRNA和蛋白表达,其中以AdipoR1和T-cad-herin的mRNA和蛋白表达量高,与AdipoR2的表达量相比差异有统计学意义(P<0.01)。结论脂联素受体1、脂联素受体2和T-钙黏蛋白,三种脂联素受体在SD大鼠乳鼠心肌细胞上均有表达,并以脂联素受体1和T-钙黏蛋白的表达为主,脂联素受体2表达量较少。

Distribution and Expression of Adiponectin Receptors in Cardiomyocytes of Sprague-Dawley Neonatal Rat

Objective To investigate the distribution and expression of adiponectin receptors(AdipoR)in cardiomyocytes of Sprague-Dawley(SD)neonatal rat.Methods Primary myocardial cells were obtained from neonatal rats by enzymatic digestion method.The molecular markerα-actin of the cardiomyocytes was observed by immunocytochemistry.The morphology of neonatal rat cardiomyocytes was studied by phase contramicroscope.The cells were cultured for 96hand the expressions of AdipoR1,AdipoR2,T-cadherin were detected by RT-PCR and immunocytochemistry.Results The mRNA and proteins of AdipoR1,AdipoR2and T-cadherin were observed in SD rat cardiomyocytes,while the expression of AdipoR1and T-cadherin were much higher than the expression of AdipoR2in cardiomyocytes(P<0.01).Conclusion AdipoR1,AdipoR2and T-cadherin were distributed in cardiomyocytes of SD rat,especially AdipoR1and T-cadherin,and expression of AdipoR2was lower.