金针菇多糖对小鼠急性肝损伤的保护作用

目的探讨金针菇多糖（polysaccharides of Flammulina velutipes,FVP）对CCl4诱导的急性肝损伤的保护作用。方法小鼠腹腔注射CCl4橄榄油溶液（0.5%,5 mL.kg^-1）12、24、48、72 h后测定血清ALT,确定CCl4急性肝损伤造模最佳时间为24 h。FVP（75,150,300 mg.kg^-1）连续口服给药8 d,第7天采用CCl4制备小鼠急性化学性肝损伤模型,24 h后测定FVP对血清ALT、AST活性,肝匀浆SOD活性、MDA含量的影响。结果 FVP能显著降低CCl4致急性肝损伤小鼠血清ALT、AST活性,提高肝匀浆SOD的活性,降低MDA的含量。结论金针菇多糖对CCl4诱发的小鼠应激性肝损伤有一定的保护作用。

The protective effects of polysaccharides of Flammulina velutipes on acute liver injury in mice

Objective The study was designed to investigate the protective effect of polysaccharides of Flammulina velutipes（FVP） against liver injury induced by CCl4.Methods When mice were intraperitoneally injected with olive solution（0.5%,5 mL·kg^-1）for 12,24,48,72 h,the plasma ALT activities were determined and the best time for CCl4-induced acute liver injury in mice was 24 h.FVP（75,150 and 300 mg·kg^-1） were orally administered to mice in experimental groups once per day for 8 days.On the seventh day,all mice were intraperitoneally injected with CCl4 to induce acute liver injury.24 hours later,all mice were sacrificed,and blood and liver were collected for measurements of ALT and AST levels,the content of MDA and the activities of SOD.Results Oral administration with FVP significantly reduced the elevated levels of ALT and AST in mice with CCl4-induced acute liver injury.The SOD level and the elevated content of MDA were lowered.Conclusion Polysaccharides of Flammulina velutipes had the protective effect against CCl4-induced acute liver injury in mice.