创伤合并海水浸泡动物感染实验研究

目的了解创伤合并海水浸泡动物细菌感染的特点。方法制作兔背部创伤动物实验模型并分成对照组和实验组。实验组致伤后放海水浸泡20 min,对照组致伤后不浸泡。用手工法和全自动微生物分析仪进行细菌学鉴定,用微生物动态快速测定系统检测血浆内毒素含量,全血白细胞数用显微镜计数法。结果创伤合并海水浸泡后,伤口如果不经过消毒处理,12 h后细菌数量显著增加,主要细菌为大肠埃希菌、鲍曼不动杆菌、创伤弧菌和金黄色葡萄球菌;血液中可检出大肠埃希菌和创伤弧菌;内毒素含量和白细胞数显著升高。结论创伤合并海水浸泡后可导致多种细菌感染,感染时间提前且程度加重。

Experimental Study on Animal Infection after Wound with Seawater Immersion

Objective To investigate the characteristics of bacterial infection after wound with seawater immersion in rabbits.Methods The wound models on skin of rabbits were made and randomly divided into two groups: control group and experimental group.The rabbits in experimental group had been immersed into seawater for 20 minutes after wound,while those in control group had not.The bacteria identification was done manually and by VITEK Auto Microbe System.Plasma endotoxin was detected by Microbiology Kinetic Rapid Reader MB-80.White blood cell count was measured by microscope counting method.Results After the wound with seawater immersion,if the wound has been treated without disinfection,the amount of bacteria increased obviously after 12h of wounded.The mainly bacteria include Escherichia coli,Acinetobacter baumannii,Vibrio vulnificus and Staphylococcus aureus.Escherichia coli and Vibrio vulnificus also can be found in the blood.The amount of endotoxin and white blood cell count also increased.Conclusion Severe bacterial infection may develop after the wound with seawater immersion.