| 高效液相色谱法测定粉刺平胶囊中黄芩苷的含量 |
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| 引用本文： | 罗世榕.高效液相色谱法测定粉刺平胶囊中黄芩苷的含量[J].海峡药学,2011,23(7):77-79. |
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| 作者姓名： | 罗世榕 |
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| 作者单位： | 福州市高新技术产业创业服务中心,福州,350002 |
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| 摘 要： | 目的建立粉刺平胶囊中黄苓苷的含量测定方法。方法采用高效液相色谱法，色谱柱为Hypersil BDS C18柱（4．6×250mm，5μm）分析柱，流动相为甲醇．0．2％磷酸溶液（39：61），检测菠长为280nm，流速为1．0mL．min^-1，柱温为30℃。结果本法精密度高．稳定性好。黄苓苷浓度在3．408～20．448μg·mL^-1范围内具有良好的线性关系（r=1）；平均加样回收率100．09％，RSD为1．55％。结论本方法简便．准确．重现性好。可作为该制剂的含量测定方法。
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| 关 键 词： | 粉刺平胶囊 黄芩苷 高效液相色谱法 |
Determination of baicalin in Fenciping capsule by HPLC |
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| LUO Shi-rong.Determination of baicalin in Fenciping capsule by HPLC[J].Strait Pharmaceutical Journal,2011,23(7):77-79. |
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| Authors: | LUO Shi-rong |
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| Affiliation: | LUO Shi-rong(Fuzhou investment service centers of high and new technological enterprises,Fuzhou 350002,China) |
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| Abstract: | OBJECTIVE To establish a method for determination of baicalin in Fenciping Capsule.METHODS Using a HPLC method with Hypersil BDS C18(4.6×250mm,5μm)column,the mobile phase was a methanol-0.2%phosphoric acid(39∶61)the detection wavelength at 280nm,flow rate was 1.0mL·min-1,and column temperature was 30℃.RESULTS The precision and stability were fine,The standard curve of baicalin was rectilinear within the range of 3.408～20.448μg·mL-1,r=1.The average recovery of baicalin was 100.09% with RSD of 1.55%.CONCLUSION The method was simple,accurate and reproducible,and can be used for the determination of baicalin. |
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| Keywords: | Fenciping Capsule Haicalin HPLC |
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