近红外漫反射光谱在脱水剂脱水作用中的应用研究

分析了脑局部皮层的近红外漫反射光谱在研究脱水剂脱水作用中的可行性。将60只SD大鼠随机分成A、B、C三组，每组分别在尾静脉推注0.9% NaCl、20%甘露醇和7.5% NaCl(均为10ml/kg)，利用双光纤微创探头实时在位采集推注前后大鼠局部脑皮质的近红外漫反射光谱，分析光谱变化。结果：A组(0.9% NaCl)推注前后光谱未见明显变化，B组(20% 甘露醇)和C组(7.5% NaCl)推注后近红外区光谱斜率绝对值的相对变化率分别上升（5.5±1.2）%和（10.5±2.5）%，三组间的差别存在统计学意义。可见，脑局部皮质的漫反射光谱可作为一种评估脱水剂脱水作用的新方法。

Application of near infrared diffusion spectrum in effect of anhydration solution

The feasibility of the near infrared diffusion spectrum of local cortex for the study of effect of anhydration solution is analyzed. 60 SD rats were divided in random into three groups A, B, C, which are treated by 0.9% NaCl, 20% Mannitol and 7.5% NaCl through vena cava respectively. The near infrared diffusion spectrum of local cortex were collected continuously before and after the injection by a bifurcated fiber probe in vivo in a mini-invasive way, Then the changing rules of the spectrum were analyzed. Few changes of the spectrum were observed in the group A(0.9% NaCl), while, the relative changes of the absolute value of the spectrum slope between near infrared range were increased by (5.5±1.2)% for group B (20% mannitol) and (10.5±2.5)% for group C (7.5% NaCl). The differences among three groups make statistic sense (p<0.05). The near infrared diffusion spectrum of local cortex can be used as a new method for estimation of effect of anhydration solution.