新疆吐鲁番雌性子午沙鼠发情周期的实验观察

目的观察子午沙鼠各个发情周期,了解子午沙鼠的发情规律。方法每天间隔4 h连续20 d采集24只雌性子午沙鼠阴道上皮细胞涂片,经苏木精-伊红染色,在显微镜下观察阴道脱落上皮细胞角化细胞形态及数量,确定子午沙鼠不同发情周期。结果子午沙鼠的发情周期可分为4个时期:发情前期(22. 0±12. 0) h,发情期(26. 8±8. 1) h,发情后期(18. 20±9. 10) h和发情间期(50. 0±38. 2) h,发情周期为(4. 7±1. 2) d。发情前期有核上皮细胞比例为(80. 20±1. 83)%;发情期角化细胞比例占(88. 00±1. 86)%;发情后期角化细胞比例(24. 80±1. 43)%、淋巴细胞比例(48. 20±2. 05)%和角化细胞比例(24. 80±1. 43)%三种细胞均存在;发情间期淋巴细胞比例为(91. 70±1. 66)%。结论雌性子午沙鼠的发情周期为(4. 7±1. 2) d,发情前期、发情期、发情后期和发情间期分别占发情周期的19. 5%、23. 8%、16. 1%和44. 3%,各期上皮细胞角化细胞形态及数量的比例明显不同。

Experimental Observation On the Estrous Cycle of Female Tyrpan Meriones meri)ianus in Xin Jiang

Objective To observe the estrous cycles of Meridian gerbils and understand the estrous regularity of Meridian gerbils. Method Twenty-four female M. meridianus vaginal epithelial cell smears were collected at intervals of 4 hours and 20 days. The morphology and quantity of conified cells of vaginal exfoliated epithelial cells were observed by hematoxylin-eosin staining. The estrous cycles of meridian gerbils were determined. Result The estrus cycle of meridian gerbils can be divided into four stages : proestrus ( 22. 0 ± 12. 0) h, estrus( 26. 8 ± 8. 1) h, metoestrus ( 18. 2 ± 9. 1 ) h and Dioestrus ( 50. 0 ± 38. 2 ) h, and eestrus cycle ( 4.7±1.2 ) d. The proportions of nucleated cellsin proestruswere ( 80.20 ±1.83 ) %.The proportions of conified cells in estrus were ( 88.00 ± 1.86 ) %.The proportions of conified cells in metoestrus were ( 24.80 ±1.43 ) %.The proportions of lymphocytes metoestrus were ( 48.20 ±2.05 ) %.The proportions of conified cells were ( 24.80 ±1.43 ) %.The proportions of lymphocytes in dioestrus were ( 91. 70±1. 66 ) %. Conclusion It provides the basis for the study of estrous cycle of female Meriones meridianus.