柚肉多糖的提取及其降血脂作用研究

目的：研究柚肉多糖（GPs）对实验性高血脂小鼠血脂含量的影响。方法：采用水提醇沉法提取GPs，葡聚糖凝胶柱层析测定其分子量。以不同剂量GPs液ig高脂模型小鼠，两周后断头取血测定正常及各高脂模型小鼠的血清脂质含量，计算其动脉硬化指数（AI）。结果：柚肉中含有分子量为27000和12000种多糖组分。与高脂模型组比较，GPs组血清中的总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-C）含量和AI均有显著降低（P〈0．01），高密度脂蛋白（HDL-C）含量则显著升高（P〈0．01）。结论：GPs有显著降脂效果，对动脉粥样硬化有一定的预防作用。

Study on the Separation and Lipid-decresing Effect of Polysaccharides in the Grapefruit

Objective:To study the effects of polysaccharides in the grapefruit(GPs)on experimental mice with hyperlipidemia.Method:The water soluble polysaccharides of grapefruit were extracted in hot water,and isolated by ethanol.GPs were purified with Sephadex-50 column chromatography.The hyperlipidemia mice were fed with different doses of GPs to determine the mice serum lipids of control,GPs and model groups after two weeks feeding and to figure out atherosclerosis index(AI).Result:The molecular weight of GPs were estimated to be(27 000) and(12 000) respectively.Compared with the model group,the concentrations of total cholesterol(TC),total triglyeride(TG),low density lipoprotein cholesterol(LDL-C) and AI in serum of GPs groups were decreased significantly(P<0.01),and the concentration of high density lipoprotein cholesterol(HDL-C)in serum was obviously increased in the meantime(P<0.01).Conclusion:The results implicate that the GPs have significantly effects in decreasing the blood lipid of hyperlipidemia and in preventing atherosclerosis.