Formation of bone-like apatite on poly(L-lactide) to improve osteoblast-like compatibility in vitro and in vivo |
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Authors: | Jiao Yanpeng Liu Zonghua Zhou Changren and Cui Fuzhai |
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Affiliation: | (1) Department of Materials Science & Engineering, Tsinghua University, Beijing, 100084, China;(2) Department of Materials Science & Engineering, Jinan University, Guangzhou, 510632, China |
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Abstract: | The biomimetic apatite coating process was adopted to modify poly(L-lactide) (PLLA) surfaces with osteoblasts-like cell compatibility.
The apatite coating was formed on the pre-hydrolyzed PLLA film and scaffold surfaces by incubating in simulated body fluid
(SBF). Scanning electron microscopy and energy dispersive X-ray analyzer were utilized to characterize the composition and
the structure of the apatite coating. The cytocompatibility of the modified PLLA films was investigated by testing osteoblast-like
attachment, proliferation, alkaline phosphatase (ALP) activity, and cell cycle. Subsequently, the modified PLLA scaffolds
were co-cultured with the osteoblasts-like in vitro and subcutaneously implanted into nude mice. The experimental results showed that the formed apatite had a nano-sized particle
and matrix configuration. The surface modification of PLLA with apatite coating significantly promoted osteoblast-like compatibility.
After a four-week culture in vivo, no significant inflammatory signs were observed in the implanted regions and osteoblast-like congeries with bone-like structure
began to form in the scaffolds. The positive results of this study suggest a good way to produce desirable PLLA biomaterials
for bone tissue engineering. |
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Keywords: | biomimatic poly(L-lactide) bone-like apatite biocompatibility |
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