序批式接触氧化反应器中细菌多样性及其功能

采用分子生物学手段，通过构建16S rDNA基因文库，对新型剩余污泥减量化处理系统——序批式生物砾间接触氧化反应器中细菌多样性进行了系统发育分析，并讨论了多种细菌共存对剩余污泥减量化的贡献.共有72个克隆子用于细菌系统发育分析. 结果表明:填料表面附着细菌与孔隙内细菌由变型菌属、噬纤维菌-屈挠杆菌-拟杆菌组(CFB)、硝化杆菌科、低G+C革兰氏阳性细菌、高G+C革兰氏阳性细菌、疣微菌科和绿菌科等七大类细菌组成. 其中，优势菌群分别是以兼具呼吸/发酵代谢方式的β变形菌纲(分别占生物膜和内泥中克隆子总量的18%和35%)和δ变形菌纲，以及以呼吸/发酵为主要代谢方式的CFB(分别占克隆子总量的24%和23%). 多种细菌对剩余污泥减量化的主要功能可归纳为能量解偶联、共代谢作用、生物溶胞作用和慢性生长种群的影响. 

Phylogenetic Diversity of Bacteria and Their Contribution to Excess Sludge Demineralization in Gravel Contact Oxidation Reactor by Batch Aerating

The phylogenetic diversity of bacteria and its function in excess sludge demineralization in a gravel contact oxidation reactor were studied by constructed 16S rDNA library, adopting molecular biology. The contribution of co-existence of multiple bacteria to excess sludge demineralization was discussed. A total of 72 16S rDNA gene clones were retrieved and analyzed phylogenetically. It was indicated that biofilm and inner sludge bacterial communities were composed of 7 species of bacteria, i.e. Proteobacteria, Cytophaga-Flexibacter-Bacteriode (CFB), Nitrobacteraceae, Low G+C, High G+C, Verrucomicrobiaceae, and Chlorobiaceae. The most abundant sequences were those related to the β-Proteobacteria group (18% clones from biofilm and 35% from inner sludge, respectively). Next were those related to the δ-Proteobacteria and CFB bacteria (24% and 23% from totalclones, respectively). Several different kinds of bacteria contributing to excess sludge decrement were associated with uncoupled metabolism, diverse bacterial interactive behavior, domination of slow growers and cryptic growth of bacteria.