| 响应面法优化白囊耙齿菌中腺苷的提取工艺 |
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| 引用本文： | 张娜,周卫,王文溪,尹秀菊,滕利荣,林凤.响应面法优化白囊耙齿菌中腺苷的提取工艺[J].中国生化药物杂志,2011,32(5). |
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| 作者姓名： | 张娜 周卫 王文溪 尹秀菊 滕利荣 林凤 |
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| 作者单位： | 吉林大学,生命科学学院,吉林,长春,130012 |
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| 基金项目： | 2009年度吉林大学“大学生创新性实验计划”资助(2009B34030) |
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| 摘 要： | 目的采用响应面分析法优化白囊耙齿菌中腺苷的提取工艺。方法在单因素实验的基础上,以提取温度、提取时间、水料比为随机因子,腺苷得率为响应值,进行三因素三水平的Box-Behnken中心组合设计,采用响应面法分析三个因素对响应值的影响,并确定最佳提取工艺条件。结果白囊耙齿菌中腺苷的最佳提取工艺条件为:提取温度39.4℃,提取时间3.4 h,料水比50.4∶1,在此条件下腺苷得率的验证值为1.09%,与预测值1.11%之间的相对误差为1.80%。结论响应面法可较好地预测白囊耙齿菌中腺苷的提取工艺。
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| 关 键 词： | 腺苷 白囊耙齿菌 响应面法 提取 |
Optimization of the extraction technique of adenosine in Irpex lacteus using the response surface methodology |
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| ZHANG Na,ZHOU Wei,WANG Wen-xi,YIN Xiu-ju,TENG Li-rong,LIN Feng.Optimization of the extraction technique of adenosine in Irpex lacteus using the response surface methodology[J].Chinese Journal of Biochemical Pharmaceutics,2011,32(5). |
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| Authors: | ZHANG Na ZHOU Wei WANG Wen-xi YIN Xiu-ju TENG Li-rong LIN Feng |
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| Affiliation: | ZHANG Na,ZHOU Wei,WANG Wen-xi,YIN Xiu-ju,TENG Li-rong,LIN Feng(School of Life Science,Jilin University,Changchun 130012,China) |
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| Abstract: | Purpose Response surface methodology(RSM)was applied to optimize the extraction conditions of adenosine in Irpex lacteus mycelium.Methods Extracting temperature,extracting time and the solvent/solid ratio were selected as causal factors.The yield of adenosine was used as the responsive values.Based on the single factors experiments,a three-factor-three-level experiments design has been developed by Box-Benhken central composite design method.The response surface method was employed to analyze the results of... |
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| Keywords: | adenosine Irpex lacteus response surface methodology extraction |
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