双歧杆菌黏附素对大鼠肠缺血-再灌注损伤的防护作用

目的:研究双歧杆菌分泌型黏附素对大鼠缺血-再灌注(I/R)后肠黏膜屏障的防护作用。方法:将54只大鼠随机分为假手术组(对照组)、模型组(I/R组)和黏附素预处理组(实验组),每组各24只。于造模成功后6 h、第1、第4和第7天,分别取6只大鼠的血和小肠标本,观察小肠组织病理改变,检测各时间点血中肿瘤坏死因子-α(TNF-α)I、L-6I、L-10、二胺氧化酶(DAO)和D-乳酸(D-LAC)的活性和含量。结果:与对照组比较I,/R组血中TNF-αI、L-6I、L-10、DAO和D-乳酸水平在各时相点均升高(P<0.05);与I/R组比较,实验组各时间点IL-6和DAO水平明显降低(P<0.05),TNF-α浓度术后第1天低于I/R组(P<0.05),术后第4和第7天实验组大鼠血浆D-LAC浓度明显低于I/R组(P<0.05),小肠病理改变较I/R组减轻(P<0.05)。结论:双歧杆菌黏附素对大鼠I/R后肠黏膜屏障具有防护作用,能减轻肠黏膜的I/R损伤。

The protection effect of bifidobacterial adhesin for ischemia reperfusion injury of intestine in rats

Objective: To investigate the protection effect of bifidobacteria adhesin on rat intestine ischemia/reperfusion(I/R) injury.Methods: Fifty-four male SD rats were randomly divided into normal group(control group,n=24),model group(I/R group,n=24) and pretreatmen group of bifidobacterial adhesin(experimental group,n=24).The rats were sacrificed at 6 h,1d,4 d and 7 d after inducing I/R model respectively by equal number.The pathological changes of the terminal ilea,and the levels of TNF-α,IL-6,IL-10,DAO,and the D-lactic acid were observed.Results: The blood level of TNF-α,IL-6,DAO and D-lactic acid in I/R group was significantly higher than control group at all time points(P<0.05),while the blood level of IL-10 was significantly lower(P< 0.05).The activity of IL-6 and DAO in experimental group was significantly lower than I/R group at all time points(P<0.05).The blood level of TNF-α in experimental group was significantly lower than I/R group.The blood level of D-LAC in experimental group was significantly lower than I/R group.Intestinal pathological damages were obviously milder in experimental group than I/R group.Conclusion: The bifidobacteria adhesin pretreatment could protect the intestinal mucosa from I/R injury and protect the small intestinal mucosal barrier.