| Akt抑制剂MK2206对乳腺癌细胞增殖和凋亡的影响 |
| |
| 引用本文： | 宋艳群,张斌,赵洪猛,曹旭晨. Akt抑制剂MK2206对乳腺癌细胞增殖和凋亡的影响[J]. 肿瘤, 2011, 31(2): 131-135. DOI: 10.3781/j.issn.1000-7431.2011.02.008 |
| |
| 作者姓名： | 宋艳群 张斌 赵洪猛 曹旭晨 |
| |
| 作者单位： | 天津医科大学附属肿瘤医院乳腺外科,天津,300060 |
| |
| 摘 要： | 目的:研究新型Akt抑制剂MK2206对体外培养的人乳腺癌T47D和MDA-MB-231细胞株增殖和凋亡的影响,并初步探讨其可能的作用机制。方法:以不同浓度的MK2206作用于人乳腺癌T47D和MDA-MB-231细胞,采用MTT法检测细胞的增殖抑制率,FCM法检测细胞的凋亡率,Western印迹法检测细胞中caspase-3、多聚ADP核糖聚合酶(polyADP-ribosepolymerase,PARP)、bcl-2、bax、磷酸化Akt(phosphorate-Akt,p-Akt)和总Akt(total-Akt,T-Akt)蛋白表达水平的变化。结果:0.1、1、10和100nmol/LMK2206作用细胞24h后,可明显抑制T47D和MDA-MB-231细胞的增殖和诱导细胞凋亡,其作用随着药物浓度的增加而增强。MK2206可促进乳腺癌细胞中caspase-3和PARP蛋白剪切,上调bax蛋白表达,下调bcl-2和p-Akt蛋白的表达,且均呈剂量依赖性,但对T-Akt表达却无明显影响。结论:MK2206可抑制乳腺癌T47D和MDA-MB-231细胞的增殖并诱导其凋亡,其机制可能与抑制Akt磷酸化从而阻断PI3K/Akt信号转导途径有关。
|
| 关 键 词： | 乳腺肿瘤 细胞增殖 细胞凋亡 蛋白激酶类 MK2206 体外研究 |
Effects of Akt inhibitor MK2206 on proliferation and apoptosis of breast cancer cells |
| |
| SONG Yan-qun,ZHANG Bin,ZHAO Hong-meng,CAO Xu-chen. Effects of Akt inhibitor MK2206 on proliferation and apoptosis of breast cancer cells[J]. Tumor, 2011, 31(2): 131-135. DOI: 10.3781/j.issn.1000-7431.2011.02.008 |
| |
| Authors: | SONG Yan-qun ZHANG Bin ZHAO Hong-meng CAO Xu-chen |
| |
| Affiliation: | SONG Yan-qun,ZHANG Bin,ZHAO Hong-meng,CAO Xu-chen Department of Breast Surgery,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China |
| |
| Abstract: | Objective:To study the effects of Akt inhibitor MK2206 on the proliferation and apoptosis of human breast cancer cell lines T47D and MDA-MB-231,and to elucidate the possible mechanism of such effects.Methods:Human breast cancer T47D and MDA-MB-231 cells were treated with different concentrations of MK2206,respectively.The inhibitory effect of MK2206 on cell proliferation was examined by MTT assay.The apoptosis rates of T47D and MDA-MB-231 cells induced by MK2206 were detected by flow cytometry(FCM).The expr... |
| |
| Keywords: | MK2206 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
