| 家兔腹腔巨噬细胞能产生单核细胞趋化蛋白-1 |
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| 引用本文： | 王国平,邓仲端,瞿智玲. 家兔腹腔巨噬细胞能产生单核细胞趋化蛋白-1[J]. 中国动脉硬化杂志, 1995, 3(4): 300-302 |
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| 作者姓名： | 王国平 邓仲端 瞿智玲 |
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| 作者单位： | 同济医科大学病理学教研室，武汉430030 |
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| 摘 要： | 用培养的兔腹有空巨噬细胞条件培养基作为趋化因子的来源，用改良的Ｂｏｙｄｅｎ小室微孔滤膜法进行单核细胞趋化试验，观察了兔腹腔巨噬细胞条件培养基对单核细胞的趋化作用和抗单核细胞趋化蛋白－１抗体对单核细胞迁移的影响，同时用Ｎｏｒｔｈｅｒｎ ｂｌｏｔ分析方法检测了ＭＣＰ－１ ｍＲＮＡ在该巨噬细胞的表达。结果显示，该条件培养基对单核细胞有明显的趋化作用，并被抗ＭＣＰ－１抗体所抑制，同时该巨噬细胞也能表达ＭＣ
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| 关 键 词： | 单核细胞 趋化蛋白－1 动脉粥样硬化 巨噬细胞 |
| 收稿时间： | 1995-10-20 |
Production of Monocyte Chemoattractant Protein-1 by in Rabbits Peritoneal Exudate Macrophages |
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| WANG Guo-Ping,DENG Zhong-Duan and QU Zhi-Ling;Monocyte chemoattractant protein- ; is a potent monocyte chemoattractant sereted by many cells.In this study,we examined if rabbit peritoneal exudate macrophages produce MCP-.. Production of Monocyte Chemoattractant Protein-1 by in Rabbits Peritoneal Exudate Macrophages[J]. Chinese Journal of Arteriosclerosis, 1995, 3(4): 300-302 |
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| Authors: | WANG Guo-Ping,DENG Zhong-Duan QU Zhi-Ling Monocyte chemoattractant protein- is a potent monocyte chemoattractant sereted by many cells.In this study,we examined if rabbit peritoneal exudate macrophages produce MCP-. |
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| Affiliation: | Department of Pathology, Tongji Medical University, Wuhan 430030,China;MCP-1 is a potent monocyte chemoattractant sereted by many cells.In this study,we examined if rabbit peritoneal exudate macrophages produce MCP-1. |
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| Abstract: | The monocyte migration induced by the media conditioned by cultured rabbit peritoneal exudate macrophages was assayed by micropore filter method using modified Boyden chamber.Influence of anti-MCP-1 antibody on monocyte migration was observed as well.Meanwhile,we examined the expression of MCP-1 mRNA in the macrophages by Northern blot analysis. Results Cultured rabbit peritoneal exudate macrophages produced a factor that was chemotactic for monocytes,and the activity due to chemotaxis and not chemokinesis,the chemotactic activity was markedly inhibited by anti-MCP-1 antibody. MCP-1 mRNA was expressed by the macrophages.Conclusions Macrophages can produce MCP-1 and play an important role in atherogenesis through attracting monocytes to migrate into subendothelial space. |
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| Keywords: | Monocyte chemoattractant protein-1 Migration Macrophage Monocyte Atherosclerosis |
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