降解长链烷烃菌株的选育及性能研究

以正十六烷无机盐培养基为选择培养基,从武汉石化输油管附近土壤中筛选出1株高效降解长链烷烃的菌株,命名C3,对其进行生理生化、16S r DNA鉴定,C3为不动杆菌属。在正十六烷浓度为1 000 mg/L的无机盐培养基中接入4%的种子液,放入35℃、125 r/min摇床中震荡60 h,C3对正十六烷的降解率可达100%,其降解动力学拟合结果符合Monod模型。将C3应用到柴蜡的降解,96 h后,1 000 mg/L的柴蜡混合溶液的降解率能达到91%。C3产生的生物表面活性剂经鉴定为磷脂类活性剂,排油圈直径为80 mm,CMC约为35 mg/L,能将水的表面张力降低到20.79 m N/m。该菌株对长链烷烃的降解提供了良好的菌源。

Study on the Selection and Degradation Characteristics of Long-chain Alkanes Degrading Bacteria

With the mineral salt medium with the n -hexadecane as the selecting medium ,a strain that can effectively de-grade the long-chain alkane is screened out from the soil near the pipeline of Wuhan Petrochemical Works ,named as C3 , conducted for physiological and biochemical identification and 16S rDNA comparison and the strain C3 is identified as Acine-tobacter sp .The n-hexadecane degradation is 100% after 4% seed liquid of the strain is inoculated on the mineral salt medi-um with 1 000 mg/L of n-hexadecane and cultured at 35 ℃ 、125 r/min for 60 h ,in accordance with Monod equation .The degradation of diesel and paraffin mixture can reach 91% after 96 h .The biosurfactants produced by strain C3 ,is identified as phospholipids ,whose oil spreading size is 80 mm ,the CMC of purified product is 35 mg/L ,and the lowest surface tension is 20 .79 mN/m .The study has provided the good resources of the microbial strain for degrading long -chain alkane .