蜜环菌菌丝体液体培养条件的优化

目的:对蜜环菌的液体培养条件进行优化,确定蜜环菌菌丝体的适宜培养条件。方法:通过测定菌丝体生物量的方法,利用液体摇瓶培养方式,筛选适宜的碳源、氮源、无机盐和生长因子,用统计学方法确定液体发酵培养基中碳源、氮源、无机盐和生长因子的最适浓度,最终确定蜜环菌的液体培养基配方。结果:碳源的最适种类为糊精,氮源最适种类为豆饼粉提取液,玉米浆、硫胺素都有促生长作用,乙醇作为小分子碳源对蜜环菌菌体生长有显著促进作用,培养基中添加硫酸铵和硝酸钠对菌体生长有抑制作用,添加磷酸二氢钾促进菌体生长。结论:通过对液体发酵培养条件优化,蜜环菌液体培养的培养基配方如下:25%的豆饼粉提取液,2%的玉米浆,2.5%的糊精,0.06%的硫胺素,1.0%的乙醇,0.3%的磷酸二氢钾,pH值6.0。以此培养基培养蜜环菌,最终菌丝体收率可达到1.9 g/100m l。

Optimization of Submerged Culture Condition for Production of Mycelial Biomass by Armillaria mellea

Objective: To optimize the submerged culture conditions for the production of mycelial biomass by Amillaria mellea.Methods: Using the statistically based experimental design in a shake flask culture,optimum concentration of each medium component was determined using the statistical method.Results: Dextrin was the suitable carbon source,bean cake extract was the suitable nitrogen source.Both corn steep liquor and thiamin favored the mycelial growth.Ethanol also favored the mycelial growth.(NH4)2SO4 and NaNO3 inhibited mycelial growth whereas KH2PO4 favored the mycelial growth.Conclusion: The optimal combination of the media concentrations for mycelial growth was as follows: bean cake extract 25%,corn steep liquor 2%,dextrin 2.5%,thiamin 0.06%,ethanol 1.0%,KH2PO4 0.3%,pH 6.0.Under the optimal culture condition,the production of mycelial biomass achieved 1.9g/100ml.