| 鼠李糖乳杆菌L7-13增菌培养基的优化 |
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| 引用本文： | 胡博,陈葙楠,梁金钟.鼠李糖乳杆菌L7-13增菌培养基的优化[J].北京工商大学学报(自然科学版),2010,28(3):32-37. |
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| 作者姓名： | 胡博 陈葙楠 梁金钟 |
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| 作者单位： | 1. 哈尔滨商业大学,食品工程学院,黑龙江,哈尔滨,150076
2. 东北林业大学,林学院,黑龙江,哈尔滨,150040 |
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| 基金项目： | 哈尔滨市"十一五"科技攻关项目 |
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| 摘 要： | 对分离到的鼠李糖乳杆菌L7-13进行增菌培养研究:采用单因素实验和正交试验的方法,通过对其吸光度和活菌数的测定来优化鼠李糖乳杆菌L7-13的培养基.结果表明:葡萄糖65 g/L、酵母粉15 g/L、牛肉粉28 g/L、大豆蛋白胨30 g/L、KH2PO43 g/L、无水乙酸钠3 g/L、柠檬酸铵2 g/L、Tween80 1.5 mL/L、MgSO4.7H2O 1.0 g/L、MnSO4.4H2O 0.5 g/L,培养28 h,所得鼠李糖乳杆菌L7-13的最大菌体密度约1010cfu/mL.此增殖优化培养基适于鼠李糖乳杆菌L7-13的生长繁殖.
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| 关 键 词： | 鼠李糖乳杆菌 菌种鉴定 增菌培养 |
OPTIMIZATION OF CULTURE MEDIUM FOR LACTOBACILLUS.RHAMNOSUS L7-13 STRAIN |
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| HU Bo,CHEN Xiang-nan and LIANG Jin-zhong.OPTIMIZATION OF CULTURE MEDIUM FOR LACTOBACILLUS.RHAMNOSUS L7-13 STRAIN[J].Journal of Beijing Technology and Business University:Natural Science Edition,2010,28(3):32-37. |
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| Authors: | HU Bo CHEN Xiang-nan and LIANG Jin-zhong |
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| Affiliation: | School of Food Engineering, Harbin University of Commerce;College of Forestry, Northeast Forestry University;School of Food Engineering, Harbin University of Commerce |
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| Abstract: | The culture medium of Lactobacillus.rhamnosus L7-13 was optimized by the single-factor test and orthogonal test using the absorbance and viable count of the fermentation broth.The optimal culture medium was as follow: 15 g/L yeast extract,28 g/L beef extract,65 g/L glucose,30 g/L soy peptone,3 g/L KH2PO4,3 g/L anhydrous sodium acetate,2 g/L ammonium citrate,1.5 mL/L Tween80,1.0 g/L MgSO4·7H2O,and 0.5 g/L MnSO4·4H2O.The cell concentration of Lactobacillus.rhamnosus L7-13 strain can reach 1010 cfu / mL using the optimal culture medium.The results show that this media was suitable for the growth and reproduction of Lactobacillus.rhamnosus L7-13 strain. |
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| Keywords: | Lactobacillus rhamnosus strain identification enriching culture |
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