丙烯醛致成年小鼠心肌细胞凋亡的作用(英文)

目的丙烯醛是一种具有高反应性的不饱和脂肪醛,它是丙烯胺的代谢产物,在体内外对各种细胞具有毒性作用。本研究探讨丙烯醛对成年小鼠心肌细胞氧自由基及钙浓度的影响及细胞凋亡的作用。方法采用改良langendorff方法分离成年小鼠心肌细胞。分别应用DCF及Fura-2 AM测定细胞内氧自由基水平及钙离子钙浓度,WST法测定心肌细胞存活率,观察凋亡DNA片段及细胞凋亡的形态变化。结果应用1μmol/L丙烯醛可明显增加心肌细胞内氧自由基水平及钙离子浓度,分别达到用药前的12倍及2倍。而且,丙烯醛引起心肌细胞损伤是一种剂量依赖性的,应用25,50及100μmol/L丙烯醛治疗的心肌细胞存活率明显低于对照组(P<0.01)。丙烯醛处理心肌细胞后,可观察到DNA断裂片段及典型的凋亡细胞形态变化。结论丙烯醛可引起成年小鼠心肌细胞的凋亡,其作用可能是由于细胞内氧自由及钙浓度的增加。

Acrolein-induced cell apoptosis in adult mice cardiomyocytes

Purpose To investigate the responsiveness of intracellular oxygen free radical and calcium on acrolein exposure and acrolein-induced cardiomyocytes apoptosis. Methods The viable adult mice cardiac myocytes were isolated by modified langendorff methods. We have examined the intracellular oxygen free radical and calcium concentration using DCF and Fura-2 AM, and the cardiomyocytes viability with WST assay. Are evaluated the DNA ladder pattern and cell apoptotic morphology on the adult mice cardiomyocytes that are exposed to acrolein. Results Our results show that acrolein can increase markedly the intracellular oxygen free radical and calcium concentration, that reach 12 fold and twofold respectively compared to the resting value when the cells were exposed to 1 μmol/L of acrolein. Moreover, the injury induced by acrolein in cardiac myocytes is concentration-dependent. The cardiomyocytes viability treated with 25, 50, 100 μmol/L of acrolein respectively were significantly lower compared to controls (P ＜ 0.01 ). DNA ladder pattern and apoptotic morphological changes were found after being exposed to acrolein in the adult mice cardiomyocytes. Conclusion It is concluded that acrolein induces adult mice cardiomyocytes apoptosis, and it may be due to the increased intracellular oxygen free radicals and calcium concentration.