铁皮石斛原球茎富硒悬浮培养条件优化

通过对铁皮石斛原球茎富硒悬浮培养的生长指标及多糖、生物碱、总蛋白质、硒含量等指标进行评价,优化铁皮石斛富硒悬浮培养条件,为开发富硒铁皮石斛产品提供理论依据与物质基础.研究结果表明,优化的铁皮石斛原球茎富硒悬浮培养基为MS+2.0 mg·L-1 6-BA+0.50 mg·L-1 NAA+30 g·L-1蔗糖+0.05 m...

Conditions Optimization of Selenium-enriched Suspension Culture for Protocorms of Dendrobium officinale

In this study， the growth index， polysaccharide， alkaloid， total protein， selenium content and other indexes of Dendrobiumcandidum Procorms Se-enriched suspension culture were evaluated to optimize the conditions of Se-enriched suspension culture of Dendrobium candidum， providing theoretical basis and material basis for the development of Se-enriched Dendrobium candidum products. The results showed that the optimal Se-enriched suspension medium for protocorms of Dendrobium officinale was MS+2.0 mg·L^-1 6-BA+ 0.50 mg·L^-1 NAA+30g·L^-1 sucrose +0.05 mg·L^-1 Na₂SeO₃， inoculation amount was 100 g·L^-1， pH was 5.8. When the subculture period was 30 days， the selenium content of protocorms was up to 4.01 mg·kg^-1， polysaccharide content was 34.79%， alkaloid content was 0.026%， and total protein content was 28.25%. The suspension culture system of selenium-enriched suspension culture for protocorms of Dendrobium officinale was established successfully.