反相高效液相法测定滋肾利肝颗粒中芍药苷的含量

目的建立一种测定滋肾利肝颗粒中芍药苷含量的反相高效液相法。方法采用Krom asil C18柱,以甲醇-0.05 mol.L-1磷酸二氢钾溶液(32∶68)为流动相,紫外检测波长230 nm,流速1 m l.m in-1。结果在所使用的色谱条件下,芍药苷出峰位置未见干扰,芍药苷在24.28~121.40μg.m l-1浓度范围内线性关系良好(r=0.999 9)。滋肾利肝颗粒中芍药苷的平均加样回收率为98.32%,相对标准偏差为1.34%(n=9)。结论该法操作简便,重现性好,可作为滋肾利肝颗粒生产上质量控制的一种可信工具。

Determination of the Content of Paeoniflorin in Zishenligan Granule by Reversed-Phase High Performance Liquid Chromatography

Objective To establish a RP-HPLC method for determination of the content of Paeoniflorin in Zishenligan Granule. Methods The analysis was carried out by using a Kromasil C18 analytical column with methanol and 0.05mol·L-1 potassium dihydrogen phosphate solution (32:68, v/v) as mobile phase. The UV detection wavelength was 230nm and the flow rate was 1ml·min-1. Results Under the chromatographic conditions used, there was no interference found in the position of Paeoniflorin peak. Paeoniflorin had fine linear responses (r=0.999 9) in the concentration range of 24.28～121.40 μg·ml-1, the average recovery of Paeoniflorin in Zishenligan Granule was 98.32% with the relative standard deviation of 1.34% (n=9). Conclusion The method employed in this analysis is convenient with good repetition and can be used as a reliable tool for quality control in the production of Zishenligan Granule.