| 大鼠脑组织蛋白质双向电泳技术的建立 |
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| 引用本文： | 周成林,王继生,李惠芝,邱宗荫,宋敏.大鼠脑组织蛋白质双向电泳技术的建立[J].重庆医科大学学报,2006,31(6):794-797. |
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| 作者姓名： | 周成林 王继生 李惠芝 邱宗荫 宋敏 |
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| 作者单位： | 1. 重庆医科大学药学院,重庆,400016
2. 重庆医科大学检验系,重庆,400016 |
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| 摘 要： | 目的：建立大鼠脑组织蛋白质双向电泳技术。方法：利用不同体积的裂解液提取大鼠脑组织中的蛋白质，并通过不同的蛋白质上样量（1mg，2mg，3mg），进行双向电泳，考马斯亮蓝染色，图谱分析。结果：在等电点3～10，分子量6．5～200ku范围内分离得到蛋白质斑点为，1mg蛋白质上样量为546个蛋白质斑点，2mg为780个，3mg为805个斑点。2mg蛋白质上样量的双向电泳图谱更清晰，分离更好。结论：成功建立了大鼠脑组织蛋白质的双向电泳技术。
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| 关 键 词： | 蛋白质组学 双向电泳 脑组织 裂解液 |
| 文章编号： | 0253-3626(2006)06-0794-04 |
| 收稿时间： | 05 16 2006 12:00AM |
| 修稿时间： | 2006年5月16日 |
Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat |
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| ZHOU Chenglin,et al.Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat[J].Journal of Chongqing Medical University,2006,31(6):794-797. |
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| Authors: | ZHOU Chenglin |
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| Affiliation: | Department of Pharmaceutical Analysis, Pharmaceutial School, Chongqing Medical University |
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| Abstract: | Objective:To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods:Lysis buffer of different volume was taken to extract brain tissue proteins of rat,and different protein quantities(1mg,2mg,3mg) were taken to establish a two-dimensional electrophoresis.Coomassie brilliant blue was applied to stain protein,and patterns were analyzed.Results:With a molecular mass between 6.5~200ku and isoelectric points(pI) from 3~10,1mg proteins obtained 546 protein spots,2mg 780,and 3mg 805.Pattern of 2mg protein was the best.Conclusion:A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully. |
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| Keywords: | Proteomics Two-dimensional electrophoresis Brain tissue Lysis buffer |
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