| 中国大陆科学钻探（CCSD）微生物研究——地下3900米一株细菌的分离与鉴定 |
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| 引用本文： | 王远亮,李光玉,东秀珠,董海良,杨克迁,董志扬.中国大陆科学钻探（CCSD）微生物研究——地下3900米一株细菌的分离与鉴定[J].岩石学报,2005,21(2):540-544. |
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| 作者姓名： | 王远亮 李光玉 东秀珠 董海良 杨克迁 董志扬 |
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| 作者单位： | 1. 中国科学院微生物研究所微生物资源国家重点实验室,北京,100080
2. 迈阿密大学地质系,俄亥俄,45056,美国 |
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| 基金项目： | 国家重点基础研究发展规划项目(2003CB716508)
国家自然科学基金重大项目(编号:40399144)
国际合作项目(2002CB713901)资助 |
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| 摘 要： | 在中国大陆科学钻探科钻一井的3911.28米处利用厌氧培养基分离到一株细菌CCSDFL3900,该菌的最适生长温度为65℃,兼性厌氧,革兰氏染色为阴性,端生芽孢,不运动,电镜观察无鞭毛。利用细菌16SrDNA通用引物对其16SrRNA进行PCR扩增,16SrRNA序列及其进化树分析结果显示,该菌为芽孢杆菌或地杆菌。生化试验检测显示,该菌株能够利用多种糖类发酵产酸,最终鉴定为热坚芽孢杆菌。
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| 关 键 词： | 中国大陆科学钻探 热坚芽孢杆菌 鉴定 分离 |
| 文章编号： | 1000-0569/2005/021(02)-0540-44 |
| 修稿时间： | 2005年1月20日 |
Microbiology research of the Chinese Continental Scientific Drilling: Isolation and identification of one bacterial strain from a 3900-meter-deep subsurface rocks |
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| WANG YuanLiang,LI GuangYu,DONG XiuZhu,DONG HaiLiang,YANG KeQian and DONG ZhiYang State Key Laboratory of Microbial Resources of Institute of Microbiology,Chinese Academy of Science,Beijing,China.Microbiology research of the Chinese Continental Scientific Drilling: Isolation and identification of one bacterial strain from a 3900-meter-deep subsurface rocks[J].Acta Petrologica Sinica,2005,21(2):540-544. |
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| Authors: | WANG YuanLiang LI GuangYu DONG XiuZhu DONG HaiLiang YANG KeQian and DONG ZhiYang State Key Laboratory of Microbial Resources of Institute of Microbiology Chinese Academy of Science Beijing China |
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| Affiliation: | WANG YuanLiang,LI GuangYu,DONG XiuZhu,DONG HaiLiang,YANG KeQian and DONG ZhiYang State Key Laboratory of Microbial Resources of Institute of Microbiology,Chinese Academy of Science,Beijing,China Department of Geology,Miami University,Oxfor,OH,USA |
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| Abstract: | A bacterial strain named CCSDFL3900 was isolated from a 3911. 28-meter-deep subsurface rock from the main borehole of Chinese Continent Scientific Drilling . It was Gram negative with no flagellalt has spores in the end, is immobile, facultative anaerobic, and grows optimally at 65 centigrade. Its 16S ribosome DNA was extracted and amplified with the universe primer of bacterial 16S ribosome DNA. Phylogenetic analysis results revealed that it belongs to the genus bacillus or geobacillus. Fermentation test further identified as it a strain of Bacillus caldotenax. |
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| Keywords: | Chinese Continent Scientific Drilling Bacillus caldotenax Identification Isolation |
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