大熊猫与金黄地鼠体外异种受精的研究

在大熊猫精子与地鼠卵的体外异种受精中,发现大熊猫精子穿入地鼠卵后可以激活受精卵产生极区,释放第二极体,受精卵内雌性原核形成。与此同时,地鼠卵的胞质也能促使大熊猫精子头发育成雄性原核,异种精卵间的相互作用与同种受精的相似。 细胞松弛素B能阻抑大熊猫雄性原核从地鼠卵皮层迁移到卵的中央,实验表明大熊猫雄性原核的迁移也受异种卵的微丝的控制。

STUDIES OF HETEROFERTILIZATION IN VITRO BETWEEN GIANT PANDA AND GOLDEN HAMSTER

The golden hamster eggs could be fertilized by giant panda sperm capacitated in vitro. The second polar body was released after spermpenetration and the ooplasm of such fertilized egg was found to be able to induce pronucleus formation. The male pronucleus formed by ho-mofertilization usually starts decondension from the periphery toward to the center of nucleus, while in heterofertilization the pronuclear decon densation process is irregular and imcomplete resulting in relative smaller size.Cytochalasin B treatment after sperm penetration prevents the male pronucleus migration from the egg cortex toward the center. It is(?) the efore, suggested that the male pronucleus migration of giant panda is also controlled by the microfilaments of the heterogeneous ooplasm.