加味滋生青阳片对自发性高血压大鼠肾脏的保护作用及机制研究

目的 观察加味滋生青阳片对自发性高血压大鼠(SHR)血压、高敏C反应蛋白(hs-CRP)、白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)及肾动脉病理学的影响,探讨其对SHR肾脏保护作用及机制.方法 将36只12周龄雄性SHR按照完全随机原则分为SHR模型组、中药组、替米沙...

Study on the Protective Effect and Mechanism of Jiawei Zisheng Qingyang Tablets on the Kidney of Spontaneously Hypertensive Rats

Objective:To observe the effects of Jiawei Zisheng Qingyang Tablets on blood pressure,high sensitivity C-reactive protein(hs-CRP),interleukin-1β(IL-1β),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)in spontaneously hypertensive rats(SHR),and to explore its renal protective effect and mechanism on SHR.Methods:A total of 36 male SHR of 12-week-old were randomly divided into the SHR model group,traditional Chinese medicine(TCM)group and telmisartan group,and WKY rats blank control group with the same number of rats were set up.The rats in the model group and the blank control group were given the same volume of sterile water by gavage for 8 weeks.The levels of hs-CRP,IL-1β,IL-10 and TNF-αin serum were measured by ELISA;the renal artery pathology was observed by HE staining.Results:The blood pressure of TCM group and telmisartan group decreased significantly after 4,6 and 8 weeks(P<0.05 or P<0.01),but there was no significant difference between the TCM group and telmisartan group(P>0.05);after 8 weeks of treatment,the plasma hs CRP,IL-1β,TNF-αin TCM group were lower than those in model group,and IL-10 was increased,while IL-1βand TNF-αin the TCM group were lower than those in telmisartan group,and the differences were statistically significant(P<0.05 or P<0.01);in telmisartan group and traditional Chinese medicine group,inflammatory infiltrating cells in renal artery wall were significantly reduced,elastic fiber arrangement was basically normal,and the situation was significantly better than that of model group.Conclusion:Jiawei Zisheng Qingyang Tablets has a significant protective effect on the kidney of SHR.The specific mechanism may be related to the decrease of blood pressure,the decrease of hs CRP,IL-1β,TNF-αand the increase of IL-10 in SHR.