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破坏ARF8基因降低拟南芥对红光的敏感性
引用本文:田长恩,周玉萍,陈琼华,陈羽中,程惠贞,雷德柱,姚焱,刘顺枝,柯德森.破坏ARF8基因降低拟南芥对红光的敏感性[J].广州大学学报(自然科学版),2009,8(4).
作者姓名:田长恩  周玉萍  陈琼华  陈羽中  程惠贞  雷德柱  姚焱  刘顺枝  柯德森
作者单位:1. 广州大学,植物抗逆基因功能研究广州市重点实验室,广东,广州,510405;广州大学,生命科学学院,广东,广州,510006
2. 广州大学,生命科学学院,广东,广州,510006
基金项目:教育部留学归国人员科研启动基金资助项目[2004]527
摘    要:通过ARF8基因的突变体arf8-1以及arf8-1与红光受体突变体phyB-5所组成的双突变体在红光条件下的表型鉴定,探讨ARF8参与拟南芥对红光的反应以及PhyB与ARF8在红光信号转导中的关系.结果表明,在红光条件下,arf8-1和phyB-5幼苗下胚轴的长度比相应野生型的长,倒伏度比相应野生型的低,而双突变体arf8-1 phyB-5的下胚轴的长度和倒伏度均比两个单突变体的更长和更低.上述结果说明,破坏ARF8基因降低了拟南芥对红光的敏感性,即ARF8参与了拟南芥对红光的反应,但没有介导PhyB所接收的红光信号转导.

关 键 词:生长素反应因子8  光敏色素B  红光敏感性  

Disruption of ARF8 reduces sensitivity to red light in Arabidopsis thaliana
TIAN Chang-en,ZHOU Yu-ping,CHEN Qiong-hua,CHEN Yu-zhong,CHENG Hui-zhen,LEI De-zhu,YAO Yan,LIU Shun-zhi,KE De-sen.Disruption of ARF8 reduces sensitivity to red light in Arabidopsis thaliana[J].Journal og Guangzhou University:Natural Science Edition,2009,8(4).
Authors:TIAN Chang-en  ZHOU Yu-ping  CHEN Qiong-hua  CHEN Yu-zhong  CHENG Hui-zhen  LEI De-zhu  YAO Yan  LIU Shun-zhi  KE De-sen
Affiliation:1.Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes;Guangzhou University;Guangzhou 510405;China;2.School of Life Sciences;Guangzhou 510006;China
Abstract:To determine whether ARF8 is involved in response to red light,we characterized the phenotype of red-light-grown seedlings of arf8-1 which is a mutant of ARF8.Also the relationship between ARF8 and PhyB(phytochrome B,a red-light receptor) has been probed through phenotypic identification of a double mutant between arf8-1 and phyB-5,a mutant line of PhyB.The results are showed as follows.Seedlings of arf8-1 and phyB-5 showed long-hypocotyl and less-disorientation phenotypes under red-light condition,suggesti...
Keywords:Auxin Response Factor 8  Phytochrome B  sensitivity to red light  
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