| 高产耐热脂肪酶嗜热脂肪地芽孢杆菌的选育 |
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| 引用本文： | 戚薇,冯艳蕊,王海宽,王建玲,邵静.高产耐热脂肪酶嗜热脂肪地芽孢杆菌的选育[J].工业微生物,2009,39(1):6-10. |
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| 作者姓名： | 戚薇 冯艳蕊 王海宽 王建玲 邵静 |
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| 作者单位： | 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津,300457 |
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| 基金项目： | 天津市自然科学基金，天津科技大学重点基金 |
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| 摘 要： | 采用氮离子注入技术对耐热脂肪酶产生菌嗜热脂肪地芽孢杆菌（Geobacillus stearothermophilus）L4进行诱变，筛选获得酶活力有较大提高且传代稳定的正突变菌株L4-3；再对L4-3进行紫外线诱变，得到脂肪酶活力提高的正突变菌株L4-3-2，其脂肪酶活力达25．71U／mL，较原始菌株M提高511．9％。高产突变株L4-3-2所产脂肪酶的最适作用温度为50℃，70℃保温60min的剩余酶活为82％，最适作用pH为7．0～8．0，为一种耐热碱性脂肪酶。
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| 关 键 词： | 耐热脂肪酶 嗜热脂肪地芽孢杆菌 氮离子注入 紫外诱变 |
Breeding of Geobacillus stearothermophilus for high producing thermostable lipase |
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| QI Wei,FENG Yan-rui,WANG Hai-kuan,WANG Jian-ling,SHAO Jing.Breeding of Geobacillus stearothermophilus for high producing thermostable lipase[J].Industrial Microbiology,2009,39(1):6-10. |
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| Authors: | QI Wei FENG Yan-rui WANG Hai-kuan WANG Jian-ling SHAO Jing |
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| Affiliation: | (Key Laboratory of Industrial Microbiology, Ministry of Education; College of Biotechnology, Tianjin University of Science ＆ Technology, Tianjin, 300457) |
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| Abstract: | A positive mutant strain L4-3 with high lipase production and genetic stability was obtained from Geobacillus stearotherrnophilus L4 by ion implantation, then another mutant strain L 4-3-2 with lipase production of 25.71 U/mL was obtained by UV irradiation on strain L4-3. The lipase activity of L 4-3-2 was 5.12 times higher than that of the parent strain （ Geobacillus stearothermophilus L4）. The lipase had an optimum activity at 50℃ and pH at 7.0-8.0. It remained 82 % activity at 70℃ for 60 min and was a thermostable alkaline lipase. |
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| Keywords: | thermostable lipase Geobacillus stearothermophilus nitrogen ion-beam injection UV induced mutation |
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