| 高效液相色谱法测定搜风理肺丸中黄芩苷的含量 |
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| 引用本文： | 吴艳,罗晓茹,王亚南,刘成红.高效液相色谱法测定搜风理肺丸中黄芩苷的含量[J].国外医学(药学分册),2009(5):373-375. |
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| 作者姓名： | 吴艳 罗晓茹 王亚南 刘成红 |
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| 作者单位： | 中国人民解放军总后勤部卫生部药品仪器检验所; |
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| 摘 要： | 目的建立搜风理肺丸中黄芩苷的含量测定方法。方法高效液相色谱法（HPLC）测定搜风理肺丸中黄芩苷的含量。采用Agilent Extend—C18（250mm×4．6mm，5μm）柱，以甲醇-0．05％磷酸水溶液（42：58V/V）为流动相，检测波长为280nm。结果黄芩苷在9．82～491．0mg·L^-1范围内，浓度与峰面积线性相关性良好（r=0．9999，n=7），平均回收率为103．34％（RSD为1．61％）。结论HPLC法测定方法简便、准确，可用于搜风理肺丸的质量控制。
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| 关 键 词： | 高效液相色谱法 搜风理肺丸 黄芩苷 含量测定 |
Determination for baicaline in Soufeng Lifei Wan by HPLC |
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| WU Yan,LUO Xiao-ru,WANG Ya-nan,LIU Cheng-hong.Determination for baicaline in Soufeng Lifei Wan by HPLC[J].Foreign Medical Sciences(Section of Pharmarcy),2009(5):373-375. |
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| Authors: | WU Yan LUO Xiao-ru WANG Ya-nan LIU Cheng-hong |
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| Affiliation: | WU Yan,LUO Xiao-ru,WANG Ya-nan,LIU Cheng-hong (Institute for Drug and Instrument Control of Health Service Department,GLD of PLA,Beijing 100071,China) |
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| Abstract: | Objective To establish the determination method for baicalin in Soufeng Lifei Wan. Methods The content of baicalin in Soufeng Lifei Wan was determined by high-performance liquid chromatography（HPLC）. We carried out the experiment with Agilent Extend-18 （250 mm × 4.6 mm,5 μm ） column and methanol-0.05% phosphoric acid water（42：58 V/V） as the mobile phase. The detective wavelength was 280 nm. Results The linear range was 9. 82 - 491.0 mg · L^ - 1 （ r = 0. 999 9, n = 7）. The average recovery percent was 103.34% （ RSD = 1.61% ）. Conclusion The HPLC method is simple and accurate, and can be used for the quality control of Soufeng Lifei Wan. |
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| Keywords: | high-performance liquid chromatography Soufeng Lifei Wan baicalin determination |
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